- Clone
- W21031N (See other available formats)
- Regulatory Status
- RUO
- Other Names
- L-selectin, LECAM-1, LAM-1, Leu-8, TQ-1
- Isotype
- Rat IgG2a, κ
-
Human peripheral blood lymphocytes were stained with anti-human CD3 (clone UCHT1) FITC and anti-human CD62L (clone W21031N) Spark Red™ 718 (left) or rat IgG2a, κ Spark Red™ 718 isotype control (right).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 384821 | 25 tests | $120.00 | |||
| 384822 | 100 tests | $240.00 | |||
CD62L is a 74-95 kD single chain type I glycoprotein referred to as L-selectin or LECAM-1. It is expressed on most peripheral blood B cells, subsets of T and NK cells, monocytes, granulocytes, and certain hematopoietic malignant cells. CD62L binds to carbohydrates present on certain glycoforms of CD34, glycam-1, and MAdCAM-1 and with a low affinity to anionic oligosaccharide sequences related to sialylated Lewis X (sLex, CD15s) through its C-type lectin domain. CD62L is important for the homing of naïve lymphocytes to high endothelial venules in peripheral lymph nodes and Peyer's patches. It also plays a role in leukocyte rolling on activated endothelial cells.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant human CD62L protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Spark Red™ 718 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Spark Red™ 718 has a maximum excitation of 697 nm and a maximum emission of 718 nm. - Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
Clone W21031N can completely block the binding of clone DREG-56 on target cells. Clone W21031N does not affect the binding of clone S21001C on target cells.
- RRID
-
AB_3106076 (BioLegend Cat. No. 384821)
AB_3106076 (BioLegend Cat. No. 384822)
Antigen Details
- Structure
- Selectin, single chain glycoprotein, 74-95 kD
- Distribution
-
Majority of B cells, naïve T cells, subset of memory T and NK cells, monocytes, granulocytes, thymocytes
- Function
- Leukocyte homing, leukocyte tethering, rolling
- Ligand/Receptor
- CD34, GlyCAM, MAdCAM-1
- Cell Type
- B cells, Granulocytes, Lymphocytes, Monocytes, Neutrophils, NK cells, T cells, Thymocytes, Tregs
- Biology Area
- Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
- Kishimoto T, et al. 1990. Proc Natl Acad Sci U S A. 87:2244-8.
- Kishimoto T, et al. 1991. Blood. 78:805-11.
- Gene ID
- 6402 View all products for this Gene ID
- UniProt
- View information about CD62L on UniProt.org
Other Formats
View All CD62L Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human CD62L | W21031N | FC |
| APC anti-human CD62L | W21031N | FC |
| Brilliant Violet 421™ anti-human CD62L | W21031N | FC |
| PE/Cyanine7 anti-human CD62L | W21031N | FC |
| APC/Cyanine7 anti-human CD62L | W21031N | FC |
| PerCP/Cyanine5.5 anti-human CD62L | W21031N | FC |
| FITC anti-human CD62L | W21031N | FC |
| PE anti-human CD62L | W21031N | FC |
| PE/Fire™ 700 anti-human CD62L | W21031N | FC |
| PE/Fire™ 810 anti-human CD62L | W21031N | FC |
| Spark Red™ 718 anti-human CD62L | W21031N | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD62L
Human peripheral blood lymphocytes were stained with purifie... -
APC anti-human CD62L
Human peripheral blood lymphocytes were stained with anti-hu... -
Brilliant Violet 421™ anti-human CD62L
Human peripheral blood lymphocytes were stained with anti-hu... -
PE/Cyanine7 anti-human CD62L
Human peripheral blood lymphocytes were stained with anti-hu... -
APC/Cyanine7 anti-human CD62L
Human peripheral blood lymphocytes were stained with anti-hu... -
PerCP/Cyanine5.5 anti-human CD62L
Human peripheral blood lymphocytes were stained with anti-hu... -
FITC anti-human CD62L
Human peripheral blood lymphocytes were stained with anti-hu... -
PE anti-human CD62L
Human peripheral blood lymphocytes were stained with anti-hu... -
PE/Fire™ 700 anti-human CD62L
Human peripheral blood lymphocytes were stained with anti-hu... -
PE/Fire™ 810 anti-human CD62L
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark Red™ 718 anti-human CD62L
Human peripheral blood lymphocytes were stained with anti-hu...