Spark Red™ 718 anti-mouse CD4 (Flexi-Fluor™) Antibody, CD4, GK1.5

Pricing & Availability

Clone: GK1.5 (See other available formats)
Regulatory Status: RUO
Other Names: L3T4, T4
Isotype: Rat IgG2b, κ

Compare all formats See Spark Red™ 718 spectral data

Cat #

Size

Price

Quantity

285218

50 µg

$288.00

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Description

CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes, a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosin kinase, lck.

Technical data sheet

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse CTL clone V4

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide

Preparation

The antibody was purified by affinity chromatography and conjugated with Spark Red™ 718 under optimal conditions.

Concentration

0.2 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

Recommended Usage

Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 10⁷ cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.

Excitation Laser

Red Laser (633 nm)

Application Notes

Additional reported applications (for the relevant formats) include: blocking of CD4⁺ T cell activation^1,4,11, thymocyte costimulation³, in vitro and in vivo depletion^2,5-8, blocking of egg-sperm cell adhesion^1,4, immunohistochemical staining of acetone-fixed frozen sections^9,10, immunoprecipitation^1,2, and spatial biology (IBEX)^12,13. The GK1.5 antibody is able to block CD4 mediated cell adhesion and T cell activation. Binding of GK1.5 antibody to CD4 T cells can be blocked by RM4-5 antibody, but not RM4-4 antibody. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100442) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Additional Product Notes

For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.

Application References (PubMed link indicates BioLegend citation)

Dialynas DP, et al. 1983. J. Immunol. 131:2445. (Block, IP)
Dialynas DP, et al. 1983. Immunol. Rev. 74:29. (IP, Deplete)
Wu L, et al. 1991. J. Exp. Med. 174:1617. (Costim)
Godfrey DI, et al. 1994. J. Immunol. 152:4783. (Block)
Gavett SH, et al. 1994. Am. J. Respir. Cell. Mol. Biol. 10:587. (Deplete)
Schuyler M, et al. 1994. Am. J. Respir. Crit. Care Med. 149:1286. (Deplete)
Ghobrial RR, et al. 1989. Clin. Immunol. Immunopathol. 52:486. (Deplete)
Israelski DM, et al. 1989. J. Immunol. 142:954. (Deplete)
Zheng B, et al. 1996. J. Exp. Med. 184:1083. (IHC)
Frei K, et al. 1997. J. Exp. Med. 185:2177. (IHC)
Felix NJ, et al. 2007. Nat. Immunol. 8:388. (Block)
Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed

RRID

AB_3662454 (BioLegend Cat. No. 285218)

Antigen Details

Structure: Ig superfamily, 55 kD
Distribution: Majority of thymocytes, T cell subset
Function: TCR co-receptor, T cell activation
Ligand/Receptor: MHC class II molecule
Cell Type: Dendritic cells, T cells, Thymocytes, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.
Gene ID: NA
UniProt: View information about CD4 on UniProt.org

Related Products

Description	Clone	Applications

Other Formats

View All CD4 Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-mouse CD4	GK1.5	FC
Biotin anti-mouse CD4	GK1.5	FC,IHC-F,ICC
FITC anti-mouse CD4	GK1.5	FC,IHC-F,ICC
PE anti-mouse CD4	GK1.5	FC
PE/Cyanine5 anti-mouse CD4	GK1.5	FC
Purified anti-mouse CD4	GK1.5	FC,IHC-F,ICC,IP,Costim,Block,Depletion
PE/Cyanine7 anti-mouse CD4	GK1.5	FC
APC/Cyanine7 anti-mouse CD4	GK1.5	FC
Alexa Fluor® 647 anti-mouse CD4	GK1.5	FC,IHC-F,ICC
Alexa Fluor® 488 anti-mouse CD4	GK1.5	FC,IHC-F,ICC
Pacific Blue™ anti-mouse CD4	GK1.5	FC
Alexa Fluor® 700 anti-mouse CD4	GK1.5	FC
PerCP anti-mouse CD4	GK1.5	FC
PerCP/Cyanine5.5 anti-mouse CD4	GK1.5	FC
Brilliant Violet 421™ anti-mouse CD4	GK1.5	FC,ICC,IHC-F
Ultra-LEAF™ Purified anti-mouse CD4	GK1.5	FC,Block,Costim,Depletion,IHC,IP
Alexa Fluor® 594 anti-mouse CD4	GK1.5	IHC-F,FC,ICC,SB
Brilliant Violet 711™ anti-mouse CD4	GK1.5	FC
Brilliant Violet 510™ anti-mouse CD4	GK1.5	FC,IHC-F,ICC
Brilliant Violet 605™ anti-mouse CD4	GK1.5	FC
Brilliant Violet 785™ anti-mouse CD4	GK1.5	FC
PE/Dazzle™ 594 anti-mouse CD4	GK1.5	FC
APC/Fire™ 750 anti-mouse CD4	GK1.5	FC
GoInVivo™ Purified anti-mouse CD4	GK1.5	FC
Brilliant Violet 750™ anti-mouse CD4	GK1.5	FC
Brilliant Violet 650™ anti-mouse CD4	GK1.5	FC
Spark Blue™ 550 anti-mouse CD4	GK1.5	FC
Spark NIR™ 685 anti-mouse CD4	GK1.5	FC
KIRAVIA Blue 520™ anti-mouse CD4	GK1.5	FC
PE/Fire™ 640 anti-mouse CD4	GK1.5	FC
APC/Fire™ 810 anti-mouse CD4	GK1.5	FC
PE/Fire™ 700 anti-mouse CD4	GK1.5	FC
Spark Violet™ 538 anti-mouse CD4	GK1.5	FC
Spark YG™ 593 anti-mouse CD4	GK1.5	FC
Spark Blue™ 574 anti-mouse CD4 Antibody	GK1.5	FC
Spark UV™ 387 anti-mouse CD4	GK1.5	FC
Spark Blue™ 515 anti-mouse CD4	GK1.5	FC
Spark PLUS UV395™ anti-mouse CD4	GK1.5	FC
Spark Red™ 718 anti-mouse CD4 (Flexi-Fluor™)	GK1.5	FC

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.