- Regulatory Status
- RUO
- Other Names
- Streptavidin- Spark Red™ 718, SAv- Spark Red™ 718, streptavidin Spark Red™ 718
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Human peripheral blood lymphocytes were stained with biotinylated anti-human CD3 (clone UCHT1) (left) or biotinylated mouse IgG1, κ isotype control (right), followed with SAV-Spark Red™ 718 and anti-human CD19 (clone HIB19) PE.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 405385 | 25 µg | $106.00 | |||
| 405386 | 100 µg | $253.00 | |||
Streptavidin is a 52.8 kDa tetrameric protein obtained from Streptomyces avidinii. It binds to biotin with a very high affinity and is one of the strongest interactions in nature with a dissociation constant of 10-14 mol/L. It is used to detect biotinylated proteins in a wide range of applications.
Product Details
- Verified Reactivity
- Human, Mouse, Rat, All Species
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- Streptavidin is conjugated with Spark Red™ 718 under optimal conditions.
- Concentration
- 0.5 mg/mL (concentration relates to the Streptavidin only component of the conjugate)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
This streptavidin product is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the Spark Red™ 718 conjugation. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Red Laser (633 nm)
Antigen Details
- Gene ID
- NA
- UniProt
- View information about Biotin on UniProt.org