Spark UV™ 387 anti-human CD66b Antibody

Pricing & Availability
Clone
G10F5 (See other available formats)
Regulatory Status
RUO
Workshop
VI MA81
Other Names
CD67, CGM6, NCA-95, CEACAM8
Isotype
Mouse IgM, κ
G10F5_Spark-UV-387_CD66b_Antibody_031924
Human peripheral blood granulocytes were stained with anti-human CD66b (clone G10F5) Spark UV™ 387 (filled histogram). Open histogram represents unstained cells.
  • G10F5_Spark-UV-387_CD66b_Antibody_031924
    Human peripheral blood granulocytes were stained with anti-human CD66b (clone G10F5) Spark UV™ 387 (filled histogram). Open histogram represents unstained cells.
Compare all formats See Spark UV™ 387 spectral data
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305127 25 tests $147.00
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305128 100 tests $323.00
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Description

CD66b is a 95-100 kD glycosylphosphatidylinositol (GPI)-linked protein also known as CD67, CGM6, and NCA-95. CD66b is a member of the immunoglobulin superfamily, carcinoembryonic antigen (CEA)-like subfamily. CD66b, expressed on granulocytes, has been reported to induce activation in neutrophils and to be involved in heterophilic adhesion with CD66c.

Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Spark UV™ 387 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark UV™ 387 has a maximum excitation of 351 nm and a maximum emission of 387 nm.

Excitation Laser
Ultraviolet Laser (355 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen, formalin-fixed paraffin-embedded tissue sections, and spatial biology (IBEX)5,6.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  3. Norling LV, et al. 2012. Arterioscler Thromb Vasc Biol. 32:1970. PubMed
  4. Meinke P, et al. 2015. Neuroimmunol Discord. 25:127. PubMed
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
RRID
AB_3106123 (BioLegend Cat. No. 305127)
AB_3106123 (BioLegend Cat. No. 305128)

Antigen Details

Structure
Ig superfamily, CEA antigen group, GPI-linked glycoprotein, 95-100 kD
Distribution

Granulocytes

Function
Cell adhesion, neutrophil activation
Ligand/Receptor
CD66c
Cell Type
Granulocytes, Neutrophils
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Kuijpers T, et al. 1993. J. Immunol. 151:4934.
2. Kuroki M, et al. 1992. J. Leuk. Biol. 52:551.

Gene ID
1088 View all products for this Gene ID
UniProt
View information about CD66b on UniProt.org
Go To Top Version: 1    Revision Date: 03/19/2024

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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