- Clone
- S20008D (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Thy-1, Thy-1.2, CD90, CD90.2, Th1.2
- Isotype
- Mouse IgG2b, κ
-
C57BL/6 mouse splenocytes were stained with anti-mouse CD3 (clone 17A2) APC/Fire™ 750 and anti-mouse CD90.2 (Thy1.2) (clone S20008D) Spark UV™ 387 (left) or stained with anti-mouse CD3 (clone 17A2) APC/Fire™ 750 only (right).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 164803 | 25 µg | $175.00 | |||
| 164804 | 100 µg | $405.00 | |||
CD90.2 is a 25-35 kD immunoglobulin superfamily member also known as Thy1.2. It is expressed on hematopoietic stem cells and neurons, all thymocytes, and peripheral T cells in Thy1.2 bearing mouse strains (Balb/c, CBA/J, C3H/He, C57BL/-, DBA, NZB/-). CD90.2 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein involved in signal transduction. CD90.2 is involved in costimulation of lymphocyte proliferation and induction of hematopoietic stem cells differentiation. CD90.2 has been shown to interact with CD45.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Mouse Thy1.2 transfected cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Spark UV™ 387 under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Spark UV™ 387 has a maximum excitation of 351 nm and a maximum emission of 387 nm. - Excitation Laser
-
Ultraviolet Laser (355 nm)
- Application Notes
-
Clone S20008D antibody reacts with mouse CD90.2 (Thy 1.2) which is expressed by C57BL/6 and BALB/C strains, but not on CD90.1 strains (AKR/J, PL, FVB, etc.).
Clone S20008D can completely block binding of clone 30-H12 on target cells.
To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with TruStain FcX™ PLUS (anti-mouse CD16/32) Antibody (Cat. No. 156603 & 156604) is recommended prior to staining. - RRID
-
AB_3106027 (BioLegend Cat. No. 164803)
AB_3106027 (BioLegend Cat. No. 164804)
Antigen Details
- Structure
- Ig superfamily, 25-35 kD
- Distribution
-
Hematopoietic stem cells and neurons, all thymocytes, peripheral T cells of the Thy-1.2 bearing mice
- Function
- Lymphocyte costimulation, proliferation and differentiation of hematopoietic stem cells
- Ligand/Receptor
- CD45
- Cell Type
- Antigen-presenting cells, B cells, Dendritic cells, Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Neurons, T cells, Thymocytes
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Death, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
- Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
- Craig W, et al. 1993. J Exp Med. 177:1331-42.
- Reif AE & Schlesinger M. 1989. Cell Surface Antigen Thy-1.
- Mayani H, et al. 1994. Blood. 83:2410-7.
- Gene ID
- 21838 View all products for this Gene ID
- UniProt
- View information about CD90.2 on UniProt.org
Other Formats
View All CD90.2 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-mouse CD90.2 (Thy1.2) | S20008D | FC |
| Spark UV™ 387 anti-mouse CD90.2 (Thy1.2) | S20008D | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-mouse CD90.2 (Thy1.2)
C57BL/6 mouse splenocytes were pre-treated with TruStain FcX... -
Spark UV™ 387 anti-mouse CD90.2 (Thy1.2)
C57BL/6 mouse splenocytes were stained with anti-mouse CD3 (...