- Regulatory Status
- RUO
-
Human peripheral blood lymphocytes were stained with biotinylated anti-human CD3, followed by SAV-Spark Ultra Violet™ 387 and anti-human CD19 APC (left) or biotinylated mouse IgG1, κ isotype control, followed with SAV-Spark Ultra Violet™ 387 and anti-human CD19 APC (right).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 405351 | 25 µg | $195.00 | |||
| 405352 | 100 µg | $425.00 | |||
Streptavidin is a 52.8 kDa tetrameric protein obtained from Streptomyces avidinii. It binds to biotin with a very high affinity and is one of the strongest interactions in nature with a dissociation constant of 10-14 mol/L. It is used to detect biotinylated proteins in a wide range of applications.
Product Details
- Verified Reactivity
- Human, Mouse, Rat, All Species
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- Streptavidin is conjugated with Spark UV™ 387 under optimal conditions
- Concentration
- 0.5 mg/mL (concentration relates to the Streptavidin only component of the conjugate)
- Storage & Handling
- The streptavidin-Spark UV™ 387 solution should be stored undiluted between 2°C and 8°C, and protected from exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this Streptavidin-Spark UV™ 387 is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the Spark UV™ 387 conjugation. For immunofluorescent staining, the recommended use of this reagent is ≤ 1 µg per 106 cells in 100 µL staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Spark UV™ 387 has a maximum excitation of 351 nm and a maximum emission of 387 nm. - Excitation Laser
-
Ultraviolet Laser (355 nm)
Antigen Details
- Gene ID
- NA
- UniProt
- View information about Biotin on UniProt.org