Spark Violet™ 423 anti-mouse CD19 Recombinant Antibody

Pricing & Availability
Clone
QA17A27 (See other available formats)
Regulatory Status
RUO
Other Names
B4
Isotype
Mouse IgG1, κ
QA17A27_SparkViolet423_CD19_Antibody_1_022422
C57BL/6 splenocytes were stained with anti-mouse CD3ɛ APC and anti-mouse CD19 recombinant (clone QA17A27) Spark Violet ™423 (left) or CD3ɛ APC only (right).
  • QA17A27_SparkViolet423_CD19_Antibody_1_022422
    C57BL/6 splenocytes were stained with anti-mouse CD3ɛ APC and anti-mouse CD19 recombinant (clone QA17A27) Spark Violet ™423 (left) or CD3ɛ APC only (right).
Compare all formats See Spark Violet™ 423 spectral data
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159811 25 µg $165.00
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159812 100 µg $347.00
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Description

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
Mouse CD19-expressing K562 human erythroleukemia cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Spark Violet™ 423 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark Violet™ 423 has a maximum excitation of 400 nm and a maximum emission of 415 nm.

Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone QA17A27 of antibody can block binding of 6D5 clone on target and vice versa.

RRID
AB_2922493 (BioLegend Cat. No. 159811)
AB_2922493 (BioLegend Cat. No. 159812)

Antigen Details

Structure
Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution

Pro-B cells to mature B cells (during development), follicular dendritic cells

Function
Modulates B cell activation and differentiation
Interaction
B cells, Dendritic cells
Ligand/Receptor
CD21, CD81, Leu-13
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References
  1. Fearon DT. 1993. Curr Opin Immunol. 5:341-8.
  2. Krop I, et al. 1996. Eur J Immunol. 26:23-42.
  3. Krop I, et al. 1996. J Immunol. 157:48-56.
  4. Tedder TF, et al. 1994. Immunol. Today 15:437-42.
Gene ID
12478 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org
Go To Top Version: 1    Revision Date: 02/24/2022

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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