Spark Violet™ 500 anti-human CD20 Antibody

Pricing & Availability
Clone
S18015E (See other available formats)
Regulatory Status
RUO
Other Names
B1, Bp35
Isotype
Mouse IgG2a, κ
S18015E_Spark-Violet-500_CD20_FC_010324
Human peripheral blood lymphocytes were stained with anti-human CD19 (clone HIB19) PE/Cyanine7 and anti-human CD20 (clone S18015E) Spark Violet™ 500 (left) or stained with anti-human CD19 (clone HIB19) PE/Cyanine7 only (right).
  • S18015E_Spark-Violet-500_CD20_FC_010324
    Human peripheral blood lymphocytes were stained with anti-human CD19 (clone HIB19) PE/Cyanine7 and anti-human CD20 (clone S18015E) Spark Violet™ 500 (left) or stained with anti-human CD19 (clone HIB19) PE/Cyanine7 only (right).
Compare all formats See Spark Violet™ 500 spectral data
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375531 25 tests $160.00
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375532 100 tests $360.00
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Description

CD20 (also known as B1 and Bp35) is a four transmembrane spanning protein measuring 33-37 kD. Expressed on pre-B cells, resting and activated B cells (not plasma cells), some follicular dendritic cells, and at low levels on a T cell subset. Involved in B-cell activation and is associated with a variety of Src family kinases.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human CD20 transfectant
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Spark Violet™ 500 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark Violet™ 500 has a maximum excitation of 393 nm and a maximum emission of 500 nm.

Excitation Laser
Violet Laser (405 nm)
RRID
AB_3097485 (BioLegend Cat. No. 375531)
AB_3097485 (BioLegend Cat. No. 375532)

Antigen Details

Structure
Four transmembrane protein (TM4SF), heavily phosphorylated after activation, 33-37 kD
Distribution

B cell, T cell subsets

Function
B cell activation
Interaction
The CD20 molecule is involved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn).
Ligand/Receptor
Src family tyrosine kinases, MHC class I, II, CD53, CD81, CD82
Cell Type
B cells, T cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References
  1. Hultin LE, et al. 1993. Cytometry 14:196.
  2. Tedder T and Engel P. 1994. Immunol. Today 15:450.
Gene ID
931 View all products for this Gene ID
UniProt
View information about CD20 on UniProt.org
Go To Top Version: 1    Revision Date: 01/03/2024

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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