- Regulatory Status
- RUO
- Other Names
- Streptavidin-Allophycocyanin, SAv-APC
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Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Sodium Citrate H.I.E.R. 1X at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of anti-human PNAd (clone MECA-79) Biotin, and anti-human CD8a (clone C8/144B) Alexa Fluor® 647 (green) at 4°C overnight, followed by 2.5 µg/mL of Streptavidin Spark YG™ 570 (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective. -
Mouse embryonic fibroblast NIH/3T3 cell line was fixed with 1% paraformaldehyde (PFA) for 10 minutes and blocked with 5% FBS for 30 minutes. Then the cells were stained with anti-mouse/human CD44 biotinylated antibody (clone IM7), followed by Streptavidin Spark YG™ 570 (red). Nuclei were counterstained with DAPI (blue). This image was captured with a 40X objective.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 405281 | 25 µg | $89.00 | |||
| 405282 | 100 µg | $206.00 | |||
Streptavidin binds to biotin with high affinity. Streptavidin-Spark YG™ 570 is useful for detecting biotinylated antibodies. The excitation of Spark YG™ 570 by 555 nm laser light induces a light emission maximum of 570 nm.
Product Details
- Verified Reactivity
- Human, Mouse, Rat, All Species
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Spark YG™ 570 under optimal conditions.
- Concentration
- 0.5 mg/mL (concentration relates to the Streptavidin only component of the conjugate)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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IHC-F - Quality tested
IHC-P, ICC - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 2.5 - 5.0 µg/mL is suggested. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 2.5 - 5.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 2.5 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
* Spark YG™ 570 has a maximum excitation of 555 nm and a maximum emission of 570 nm.
Antigen Details
- Gene ID
- NA
- UniProt
- View information about Biotin on UniProt.org