Spark YG™ 581 anti-human IgD Antibody

Pricing & Availability
Clone
W18340F (See other available formats)
Regulatory Status
RUO
Other Names
Ig delta chain C region
Isotype
Rat IgG2a, κ
W18340F_SPKYG581_IgD_Antibody_122120.png
Human peripheral blood lymphocytes were stained with anti-human CD19 Brilliant Violet 421™ and anti-human IgD (clone W18340F) Spark YG™ 581 (left), or stained with anti-human CD19 Brilliant Violet 421™ only (right).
  • W18340F_SPKYG581_IgD_Antibody_122120.png
    Human peripheral blood lymphocytes were stained with anti-human CD19 Brilliant Violet 421™ and anti-human IgD (clone W18340F) Spark YG™ 581 (left), or stained with anti-human CD19 Brilliant Violet 421™ only (right).
Compare all formats See Spark YG™ 581 spectral data
Cat # Size Price Quantity Check Availability
307805 25 tests $159.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
307806 100 tests $358.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

IgD, a member of the immunoglobulin (Ig) family, is expressed in naïve B cells. It has 3 Ig-like domains and exists in a transmembrane and a soluble form. In general, IgD is not secreted and usually its expression is lost after the Ig isotype switch. After antigen binding, IgD signals through the CD79a/CD79b (Igα/Igβ) heterodimer, resulting in the activation of the B cell. (BioLegend Website)

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Native human IgD protein (full length)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Spark YG™ 581 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark YG™ 581 has a maximum excitation of 562 nm and a maximum emission of 581 nm.

Excitation Laser
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

This clone does not cross react with mouse tissues.

RRID
AB_2890743 (BioLegend Cat. No. 307805)
AB_2890743 (BioLegend Cat. No. 307806)

Antigen Details

Distribution

Naïve B cells

Function
Antigen binding, B cell activation
Cell Type
B cells
Biology Area
Immunology
Antigen References
  1. Geisberger R, et al. 2006. Immunology. 118:429-37.
  2. Weller S, et al. 2005. Eur J Immunol. 35:2789-92.
  3. Brandtzaeg P & Johansen FE. 2005. Immunol Rev. 206:32-63.
Gene ID
3495 View all products for this Gene ID
UniProt
View information about IgD on UniProt.org
Go To Top Version: 1    Revision Date: 12/21/2020

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login/Register
Forgot your password? Reset Password
Request an Account