TMB High Sensitivity Substrate Solution

Pricing & Availability
Regulatory Status
RUO
Other Names
TMB HS Substrate
Cat # Size Price Quantity Check Availability
421501 60 mL $30.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

The TMB High Sensitivity (TMB HS) Substrate solution is an one-step working solution for HRP (horse-radish peroxidase) labeled conjugates. TMB HS substrate solution provides high signal and high sensitivity for ELISA. It is recommended for cytokine ELISA reagent sets. The substrate can be catalyzed with peroxidase to produce a pale blue color which can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2N H2SO4 or 1M H3PO4 (resulting in a yellow color), and read at 450 nm.

Technical data sheet

Product Details

Storage & Handling
The TMB High Sensitivity Substrate Solution should be stored between 2°C and 8°C. Avoid prolonged exposure to light, contact with metal, air, or extreme temperatures.
Application

ELISA

Application References

(PubMed link indicates BioLegend citation)
  1. Zeng S, et al. 2008. Int Immunopharmacol. 10:1707. PubMed
  2. Eisenach PA, et al. 2010. J. Cell Sci. 123:4182. PubMed
  3. Fan YY, et al. 2012. J Lipid Res. 53:1287. PubMed
  4. Querol L, et al. 2014. Neurology. 82:879. PubMed
  5. Kiianitsa K, et al. 2104. Nucleic Acids Res. 42:108. PubMed
  6. Nogales A, et al. 2014. J Virol. 88:10525. PubMed
Product Citations
  1. Querol L, et al. 2014. Neurology. 82:879. PubMed
  2. Fan Y, et al. 2012. J Lipid Res. 53:1287. PubMed
  3. Cho M, et al. 2016. J Immunol. 197: 1065 - 1073. PubMed
  4. Nogales A, et al. 2017. J Virol. 10.1128/JVI.00720-17. PubMed
  5. Zeng S, et al. 2008. Int Immunopharmacol. 10:1707. PubMed
  6. Sheppard S, et al. 2017. Nat Commun. 8:13930. PubMed
  7. Maizels K 2014. Nucleic Acids Res. 42:108. PubMed
  8. Eisenach P, et al. 2010. J Cell Sci. 123:4182. PubMed
  9. Sinha D, et al. 2020. Anal Biochem. 113827:608. PubMed
  10. Nogales A, et al. 2014. J Virol. 88:10525. PubMed
  11. Rother N, et al. 2017. Front Immunol. 10.3389/fimmu.2017.01136. PubMed

Antigen Details

Gene ID
NA
Go To Top Version: 1    Revision Date: 11/30/2012

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

Login/Register
Forgot your password? Reset Password
Request an Account