- Clone
- 29E.2A3 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Programmed cell death ligand 1 (PD-L1), B7 homolog 1 (B7-H1)
- Isotype
- Mouse IgG2b, κ
- Barcode Sequence
- GTTGTCCGACAATAC
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 329749 | 10 µg | $369.00 | |||
CD274, also known as PD-L1 and B7-H1, is type I transmembrane glycoprotein that serves as a ligand for CD279 (PD-1). This interaction is believed to regulate the balance between the stimulatory and inhibitory signals needed for responses to microbes and maintenance of self-tolerance. CD274 is involved in the costimulation of T cell proliferation and IL-10 and IFN-γ production in an IL-2-dependent and CD279-independent manner. Conflicting data has shown that CD274 can inhibit T cell proliferation and cytokine production, and alternatively, enhance T cell activation. Other studies suggest that CD274 may signal bidirectionally, raising interesting implications for its expression in a wide variety of cell types, including T and B cells, antigen-presenting cells, and nonhematopoietic cells.
Product Details
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Full length human PD-L1
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA
- Preparation
- The antibody was purified by chromatography and conjugated with TotalSeq™-B oligomer under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
PG - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-B antibodies are compatible with 10x Genomics Single Cell Gene Expression Solutions.
To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions.
Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform. - Application Notes
-
Clone 29E.2A3 is reported to recognize an epitope on PD-L1 within the PD-L1-CD80 binding region5. Additional reported applications (for the relevant formats) include: blocking1-3 and immunohistochemical staining of acetone-fixed frozen sections1. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 329715, 329716, 329745 - 329748).
It has been observed that clone 29E.2A3 is able to bind to Alexa Fluor® 700 antibody conjugates during multi-color immunofluorescent staining. This interaction can be resolved by sequentially staining with the 29E.2A3 antibody first and then followed by the Alexa Fluor® 700 conjugate of interest.Clone 29E.2A3 does not work in Western blot applications7.
- Additional Product Notes
-
TotalSeq™ reagents are designed to profile protein levels at a single cell level following an optimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g. 10x Genomics Chromium System and Reagents) and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq.
The barcode flanking sequences are GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTNNNNNNNNNN (PCR handle), and NNNNNNNNNGCTTTAAGGCCGGTCCTAGC*A*A (capture sequence). N represents either randomly selected A, C, G, or T, and * indicates a phosphorothioated bond, to prevent nuclease degradation.
View more applications data for this product in our Scientific Poster Library. -
Application References
(PubMed link indicates BioLegend citation) -
- Brown J, et al. 2003. J. Immunol. 170:1257. (FC, IHC, Block)
- Radziewicz H, et al. 2007. J. Virol. 81:2545. (Block)
- Nakamoto N, et al. 2009. PLoS Pathog. 5:e1000313. (Block)
- Barsoum IB, et al. 2014. Cancer Res. 74:665. PubMed
- Haile, S et al. 2013. J. Immunol. 191:2829.
- RL M, et al. 2015. PNAS. 112:6506-6514. PubMed
- Mahoney KM, et al. 2015. Cancer Immunol. Res. 3:1308.
- RRID
-
AB_2800859 (BioLegend Cat. No. 329749)
Antigen Details
- Distribution
-
T cells, B cells, NK cells, monocytes/macrophages, granulocytes and dendritic cells
- Function
- CD274 is involved in the costimulatory signal, essential for T lymphocyte proliferation and production of IL-10 and IFN-γ, in an IL-2-dependent and a PD-1-CD1-independent manner. Its interaction with PD-1-CD1 inhibits T-cell proliferation and cytokine production.
- Ligand/Receptor
- PD-1 (PDCD1)
- Cell Type
- B cells, Dendritic cells, Fibroblasts, Granulocytes, Macrophages, Monocytes, NK cells, T cells
- Biology Area
- Cancer Biomarkers, Costimulatory Molecules, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules, Immune Checkpoint Receptors
- Antigen References
-
1. Sharpe A, et al. 2007. Nat. Immunol. 8:239.
- Gene ID
- 29126 View all products for this Gene ID
- UniProt
- View information about CD274 on UniProt.org
Other Formats
View All CD274 Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD274 (B7-H1, PD-L1)
PHA-stimulated (3 days) human peripheral blood lymphocytes w... 
Human paraffin embedded tonsil tissue stained with purified ... -
Biotin anti-human CD274 (B7-H1, PD-L1)
Human peripheral blood lymphocytes stained with biotinylated... -
PE anti-human CD274 (B7-H1, PD-L1)
PHA-stimulated (3 days) human peripheral blood lymphocytes w... -
APC anti-human CD274 (B7-H1, PD-L1)
PHA-stimulated (3 days) human peripheral blood lymphocytes w... -
Brilliant Violet 421™ anti-human CD274 (B7-H1, PD-L1)
PHA-stimulated (3 days) human peripheral blood lymphocytes w... -
Ultra-LEAF™ Purified anti-human CD274 (B7-H1, PD-L1)
PHA-stimulated (3 days) human peripheral blood lymphocytes w... -
PE/Cyanine7 anti-human CD274 (B7-H1, PD-L1)
PHA-stimulated (3 days) human peripheral blood lymphocytes w... 
Human peripheral blood lymphocytes were stained with CD274 (... -
Purified anti-human CD274 (B7-H1, PD-L1) (Maxpar® Ready)
Human PBMCs were incubated for three days in media alone (to... 
-
Brilliant Violet 711™ anti-human CD274 (B7-H1, PD-L1)
PHA-stimulated (3 days) human peripheral blood lymphocytes w... -
Brilliant Violet 605™ anti-human CD274 (B7-H1, PD-L1)
PHA-stimulated (three days) human peripheral blood mononucle... -
GoInVivo™ Purified anti-human CD274 (B7-H1, PD-L1)
-
PE/Dazzle™ 594 anti-human CD274 (B7-H1, PD-L1)
Human peripheral blood lymphocytes were activated for 3 days... 
-
Brilliant Violet 785™ anti-human CD274 (B7-H1, PD-L1)
PHA-stimulated (3 days) human peripheral blood lymphocytes w... -
Brilliant Violet 510™ anti-human CD274 (B7-H1, PD-L1)
PHA-stimulated (3 days) human peripheral blood lymphocytes w... -
PerCP/Cyanine5.5 anti-human CD274 (B7-H1, PD-L1)
PHA-stimulated (3 days) human peripheral blood lymphocytes w... -
Brilliant Violet 650™ anti-human CD274 (B7-H1, PD-L1)
PHA-stimulated (3 days) human peripheral blood mononuclear c... -
Alexa Fluor® 594 anti-human CD274 (B7-H1, PD-L1)
Human paraffin embedded tonsil tissue was stained with Alexa... -
TotalSeq™-A0007 anti-human CD274 (B7-H1, PD-L1)
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TotalSeq™-B0007 anti-human CD274 (B7-H1, PD-L1)
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TotalSeq™-C0007 anti-human CD274 (B7-H1, PD-L1)
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TotalSeq™-D0007 anti-human CD274 (B7-H1, PD-L1)
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PE/Fire™ 810 anti-human CD274 (B7-H1, PD-L1) Antibody
PHA-stimulated (3 days) human peripheral blood mononuclear c... -
PE/Cyanine5 anti-human CD274 (B7-H1, PD-L1)
PHA-stimulated (3 days) human peripheral blood mononuclear c... -
Spark YG™ 570 anti-human CD274 (B7-H1, PD-L1)
PHA-stimulated (3 days) human peripheral blood lymphocytes w... 
Human paraffin-embedded tonsil tissue slices were prepared w...