- Clone
- QA18A28 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, GZMB, CCP1, Asp-aseGranzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, GZMB, CCP1, Asp-ase
- Isotype
- Rat IgG1, κ
- Barcode Sequence
- GCGTGTTGTGGTATT
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 396429 | 10 µg | $369.00 | |||
Granzyme B is a 32 kD serine protease, also known as granzyme-2, serine protease B, CCP1, Asp-ase, and CTLA-1. Granzyme B is abundantly stored in the granules of cytotoxic T lymphocytes and NK cells. Low level of expression has been reported in granulocytes, B cells, and activated dendritic cells. Granzyme B is crucial for rapid induction of cell death and apoptosis through interaction with mannose-6-phosphate receptor.
Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Recombinant
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA
- Preparation
- The antibody was purified by chromatography and conjugated with TotalSeq™-B oligomer under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
ICPG - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™- B antibodies are compatible with 10x Genomics Single Cell Gene Expression Solutions.
To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 0.5 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions.
Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform. - Application Notes
-
Clone QA18A28 is compatible with Fixation buffer (Cat. No. 420801) and Intracellular Staining Permeabilization Wash Buffer (Cat. No. 421002) in both C57BL/6 splenocytes and human PBMC. QA18A28 is also compatible with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401).
- Additional Product Notes
-
TotalSeq™ reagents are designed to profile protein levels at a single cell level following an optimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g. 10x Genomics Chromium System and Reagents) and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq.
The barcode flanking sequences are GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTNNNNNNNNNN (PCR handle), and NNNNNNNNNGCTTTAAGGCCGGTCCTAGC*A*A (capture sequence). N represents either randomly selected A, C, G, or T, and * indicates a phosphorothioated bond, to prevent nuclease degradation.
View more applications data for this product in our Scientific Poster Library. - RRID
-
AB_3068304 (BioLegend Cat. No. 396429)
Antigen Details
- Structure
- 32 kD serine protease
- Distribution
-
Cytotoxic T cells, NK cells, and neutrophils, low on granulocytes, B cells and activated dendritic cells
- Function
- Granzyme B is able to induce target cell apoptosis by activating caspase independent pathways. Granzyme B is induced in CD8+ T lymphocytes with ConA/ IL-2 and CD4+ T lymphocytes with anti CD3/CD28 or CD3/CD46.
- Interaction
- Caspase-3
- Ligand/Receptor
- Mannose-6-phosphate receptor
- Cell Type
- Neutrophils, T cells
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroscience
- Molecular Family
- Enzymes and Regulators, Proteases
- Antigen References
-
- Estebanez-Perpina E, et al. 2000. Biol Chem. 381:1203.
- Griffiths GM. And S. Isaaz, et al. 1993. J. Cell Biol. 120:885.
- Spaeny-Dekking EH, et al. 1998. J. Immunol. 160:3610.
- Wagner C, et al. 2008. Mol. Immunol. 45:1761.
- Gene ID
- 3002 View all products for this Gene ID 14939 View all products for this Gene ID
- UniProt
- View information about Granzyme B on UniProt.org
Other Formats
View All Granzyme B Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-human/mouse Granzyme B Recombinant Antibody
Human peripheral blood mononuclear cells were stained with C... 
C57BL/6 mouse splenocytes were stimulated with plate-bound a... -
FITC anti-human/mouse Granzyme B Recombinant Antibody
Human peripheral blood mononuclear cells were stained with C... -
APC anti-human/mouse Granzyme B Recombinant Antibody
Human peripheral blood mononuclear cells were stained with C... -
PE/Cyanine7 anti-human/mouse Granzyme B Recombinant Antibody
Human peripheral blood mononuclear cells were stained with C... -
Brilliant Violet 421™ anti-human/mouse Granzyme B Recombinant Antibody
Human peripheral blood mononuclear cells were stained with C... -
PerCP/Cyanine5.5 anti-human/mouse Granzyme B Recombinant Antibody
Human peripheral blood mononuclear cells were stained with C... -
Purified anti-human/mouse Granzyme B Recombinant Antibody
Human peripheral blood mononuclear cells were fixed and perm... -
PerCP anti-human/mouse Granzyme B Recombinant Antibody
Human peripheral blood mononuclear cells were stained with a... -
APC/Fire™ 750 anti-human/mouse Granzyme B Recombinant Antibody
Human peripheral blood mononuclear cells were stained with a... -
Pacific Blue™ anti-human/mouse Granzyme B Recombinant Antibody
Human peripheral blood mononuclear cells were stained with a... -
Alexa Fluor® 647 anti-human/mouse Granzyme B Recombinant Antibody
Human peripheral blood mononuclear cells were stained with a... -
Alexa Fluor® 488 anti-human/mouse Granzyme B Recombinant Antibody
Human peripheral blood mononuclear cells were stained with a... -
Alexa Fluor® 700 anti-human/mouse Granzyme B Recombinant Antibody
Human peripheral blood mononuclear cells were stained with a... -
PE/Dazzle™ 594 anti-human/mouse Granzyme B Recombinant Antibody
Human peripheral blood mononuclear cells were stained with a... -
TotalSeq™-B1116 anti-human/mouse Granzyme B Recombinant Antibody
-
Brilliant Violet 510™ anti-human/mouse Granzyme B Recombinant Antibody
Human peripheral blood mononuclear cells were surface staine... -
Brilliant Violet 605™ anti-human/mouse Granzyme B Recombinant Antibody
Human peripheral blood mononuclear cells were surface staine... -
Brilliant Violet 650™ anti-human/mouse Granzyme B Recombinant Antibody
Human peripheral blood mononuclear cells were surface staine... -
Brilliant Violet 785™ anti-human/mouse Granzyme B Recombinant Antibody
Human peripheral blood mononuclear cells were surface staine... -
Brilliant Violet 750™ anti-human/mouse Granzyme B Recombinant Antibody
Human peripheral blood mononuclear cells were stained with a... -
Spark UV™ 387 anti-human/mouse Granzyme B Recombinant Antibody
Human peripheral blood mononuclear cells were stained with a...