TotalSeq™-B1164 anti-human IL-10 Antibody

Product Details

Verified Reactivity

Human

Reported Reactivity

Baboon, Rhesus, Cynomolgus

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

COS - expressed, recombinant human IL-10

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA

Preparation

The antibody was purified by chromatography and conjugated with TotalSeq™-B oligomer under optimal conditions.

Concentration

0.5 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

ICPG - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™- B antibodies are compatible with 10x Genomics Single Cell Gene Expression Solutions.

To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions. 

Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform.

Application Notes

ELISA Capture^1-5 or ELISPOT Capture⁶: The Ultra-LEAFpurified JES3-9D7 antibody is useful as the capture antibody in a sandwich ELISA, when used in conjunction with the biotinylated JES3-12G8 antibody (Cat. No. 501502) as the detecting antibody and recombinant human IL-10 (Cat. No. 571009) as the standard. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture.
Neutralization^1-3,9: The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IL-10 bioactivity (Cat. Nos. 501427 & 501428). The JES3-9D7 antibody can neutralize the bioactivity of natural or recombinant IL-10.
Additional reported applications (for the relevant formats) include: immunohistochemical staining¹².
Note: For testing human IL-10 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. Nos. 430601 & 430606) are specially developed and recommended.

The JES3-9D7 antibody reacts with human and viral interleukin-10 (IL-10).

Additional Product Notes

TotalSeq™ reagents are designed to profile protein levels at a single cell level following an optimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g. 10x Genomics Chromium System and Reagents) and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq.

The barcode flanking sequences are GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTNNNNNNNNNN (PCR handle), and NNNNNNNNNGCTTTAAGGCCGGTCCTAGC*A*A (capture sequence). N represents either randomly selected A, C, G, or T, and * indicates a phosphorothioated bond, to prevent nuclease degradation.

View more applications data for this product in our Scientific Poster Library.

Application References

(PubMed link indicates BioLegend citation)

1. Abrams J, et al. 1992. Immunol. Rev. 127:5. (ELISA Capture, Neut)
2. Gotlieb W, et al. 1992. Cytokine 4:385. (ELISA Capture, Neut)
3. Yssel H, et al. 1992. J. Immunol. 149:2378. (ELISA Capture, Neut)
4. Abrams J. 1995. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.20. (ELISA Capture)
5. Burdin N, et al. 1993. J. Exp. Med. 177:295. (ELISA Capture)
6. Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.19. (ELISPOT Capture)
7. Schaerli P, et al. 2000. J. Exp. Med. 192:1553.
8. Jason J, et al. 1999. Clin. Diagn. Lab Immunol. 6:73.
9. Akdis CA, et al. 1998. J. Clin. Invest. 102:98. (Neut)
10. Stary G, et al. 2011. J. Immunol. 186:103. PubMed
11. Mason GM, et al. 2012. PNAS. PubMed
12. 12. Smith DR, et al. 1994. Am. J. Pathol. 145:18. (IHC)

RRID

AB_3068181 (BioLegend Cat. No. 501429)

Disclaimer

The use of TotalSeq™-B antibodies against intracellular proteins has not been validated by 10x Genomics. 10x Genomics will not provide support to customers using this product. The quality of gene expression data is likely to be negatively impacted when using this product and its protocol. BioLegend does not guarantee high quality gene expression data with its use. BioLegend is not able to provide sample datasets, user optimization is required.

Antigen Details

Structure

Acid-labile cytokine, dimer, 35-40 kD (Mammalian)

Bioactivity

Inhibit IFN-γ, TNF-β, IL-2 production by T_H1 clones; inhibits macrophage-mediated IL-1, IL-6, TNF-α synthesis; suppress delayed type hypersensitivity response; stimulate T_H2 cell response; mast cell proliferation in

Cell Sources

Activated CD8⁺ and CD4⁺ T cells, activated monocytes, mast cells, Ly-1 B (mouse)

Cell Targets

T cells, B cells, mast cells, macrophages

Receptors

IL-10R (CDw210)

Cell Type

Tregs

Biology Area

Cell Biology, Immunology, Neuroinflammation, Neuroscience

Molecular Family

Cytokines/Chemokines

Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. de Waal-Malefyt R, et al. 1992. Curr. Opin. Immunol. 4:314.
3. Howard M, et al. 1992. Immunol. Today. 13:198.
4. Quesniaux V. 1992. Research Immunol. 143:385.

Regulation

Production inhibited by IL-4, IL-10

Gene ID

3586 View all products for this Gene ID

UniProt

View information about IL-10 on UniProt.org

Related Protocols

• TotalSeq™-B Surface and Intracellular Protein Staining with 10x Single Cell 3' Reagent Kit v3.1

Other Formats