TotalSeq™-Bn1386 anti-human CD19 Antibody

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Rabbit

Immunogen

Recombinant Human CD19 protein

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA

Preparation

The antibody was purified by chromatography and conjugated with TotalSeq™-Bn oligomer under optimal conditions.

Concentration

0.5 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

SB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining in formalin-fixed, paraffin-embedded (FFPE) lymphoid tissue, and the oligomer sequence is confirmed by sequencing. TotalSeq™-Bn antibodies are compatible with the 10x Visium CytAssist Gene and Protein Expression Assay.

To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution at 14,000xg at 2 − 8°C for 10 minutes before use. Carefully pipette out the liquid avoiding the bottom of the tube when handling. To determine and optimize dilutions for the addition of Totalseq™-Bn antibodies into pre-designed antibody panels, refer to 10x Genomics Custom Add-on Antibody Optimization guide.

Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform.

Additional Product Notes

TotalSeq™-Bn reagents are designed to profile protein levels following an optimized protocol in spatial transcriptomics. Compatible spatial biology devices (e.g. Imaging System, 10x Genomics Visium Spatial CytAssist Gene and Protein Expression instruments and reagents) and sequencer (e.g. Illumina analyzers) are required. TotalSeq™-B reagents are not compatible with the 10x Genomics Visium system. The complete barcode sequence may be provided upon request. Please contact technical support for more information, or visit TotalSeq™-Bn Reagents for 10x Genomics Visium CytAssist Gene and Protein Assay.

RRID

AB_3105974 (BioLegend Cat. No. 385303)

Antigen Details

Structure

Ig superfamily, type I transmembrane glycoprotein, 95 kD

Distribution

B lineage (except plasma cells), follicular dendritic cells

Function

B cell activation and differentiation

Ligand/Receptor

Forms complex with CD21 (CR2) and CD81 (TAPA-1), BCR coreceptor

Cell Type

B cells, Dendritic cells

Biology Area

Costimulatory Molecules, Immunology

Molecular Family

CD Molecules

Antigen References

Tedder T, et al. 1994. Immunol Today. 15:437-42.
Bradbury L, et al. 1993. J Immunol. 151:2915-27.

Gene ID

930 View all products for this Gene ID

UniProt

View information about CD19 on UniProt.org

Other Formats