- Clone
- 104 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Ly-5.2, LCA
- Isotype
- Mouse (SJL) IgG2a, κ
- Barcode Sequence
- CACCGTCATTCAACC
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 109855 | 10 µg | $369.00 | |||
CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- B10.S mouse thymocytes and splenocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA
- Preparation
- The antibody was purified by chromatography and conjugated with TotalSeq™-C oligomer under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
PG - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-C antibodies are compatible with 10x Genomics Chromium Single Cell Immune Profiling Solution.
To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions.
Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform. - Application Notes
-
The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3.
- Additional Product Notes
-
TotalSeq™ reagents are designed to profile protein levels at a single cell level following an optimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g. 10x Genomics Chromium System and Reagents) and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq.
The barcode flanking sequences are CGGAGATGTGTATAAGAGACAGNNNNNNNNNN (PCR handle), and NNNNNNNNNCCCATATAAGA*A*A (capture sequence). N represents either randomly selected A, C, G, or T, and * indicates a phosphorothioated bond, to prevent nuclease degradation.
View more applications data for this product in our Scientific Poster Library. -
Application References
(PubMed link indicates BioLegend citation) -
- Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
- Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
- Suzuki K, et al. 2000. Immunity 13:691. (IHC)
- Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
- Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
- Pascal V, et al. 2007. J. Immunol. 179:1751.
- Burman AC, et al. 2007. Blood 110:1064.
- Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
- Phan TG, et al. 2007. Nature Immunol. 8:992.
- Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
- Wen T, et al. 2013. PNAS. 110:6067. PubMed
- Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
- RRID
-
AB_2819821 (BioLegend Cat. No. 109855)
Antigen Details
- Structure
- Protein tyrosine phosphatase (PTP) family, 180-240 kD
- Distribution
-
All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice
- Function
- Phosphatase, T and B cell activation
- Ligand/Receptor
- Galectin-1, CD2, CD3, CD4
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules
- Antigen References
-
1. Suzuki K, et al. 2000. Immunity 13:691.
- Gene ID
- 19264 View all products for this Gene ID
- UniProt
- View information about CD45.2 on UniProt.org
Other Formats
View All CD45.2 Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Biotin anti-mouse CD45.2
C57BL/6 mouse splenocytes stained with biotinylated 104, fol... -
FITC anti-mouse CD45.2
C57BL/6 mouse splenocytes stained with 104 FITC -
PE anti-mouse CD45.2
BALB/c (solid line) and SJL (broken line) splenocytes staine... -
Purified anti-mouse CD45.2
C57BL/6 mouse splenocytes stained with purified 104, followe... -
APC anti-mouse CD45.2
C57BL/6 mouse splenocytes stained with 104 APC -
Alexa Fluor® 488 anti-mouse CD45.2
C57BL/6 mouse splenocytes stained with 104 Alexa Fluor® 488 -
Alexa Fluor® 647 anti-mouse CD45.2
C57BL/6 mouse splenocytes stained with 104 Alexa Fluor® 647 -
Pacific Blue™ anti-mouse CD45.2
C57BL/6 mouse splenocytes stained with Pacific Blue™ 1... -
Alexa Fluor® 700 anti-mouse CD45.2
C57BL/6 mouse splenocytes stained with 104 Alexa Fluor® ... -
APC/Cyanine7 anti-mouse CD45.2
C57BL/6 mouse splenocytes were stained with CD45.2 (clone 10... -
PerCP anti-mouse CD45.2
C57BL/6 mouse splenocytes stained with 104 PerCP -
PerCP/Cyanine5.5 anti-mouse CD45.2
C57BL/6 mouse splenocytes stained with 104 PerCP/Cyanine5.5 -
PE/Cyanine7 anti-mouse CD45.2
C57BL/6 mouse splenocytes stained with 104 PE/Cyanine7 -
Brilliant Violet 421™ anti-mouse CD45.2
C57BL/6 mouse splenocytes were stained with CD45.2 (clone 10... -
Brilliant Violet 570™ anti-mouse CD45.2
C57BL/6 mouse splenocytes were stained with CD45.2 (clone 10... -
Brilliant Violet 650™ anti-mouse CD45.2
C57BL/6 mouse splenocytes were stained with CD45.2 (clone 10... -
Brilliant Violet 510™ anti-mouse CD45.2
C57BL/6 mouse splenocytes were stained with CD45.2 (clone 10... -
Brilliant Violet 785™ anti-mouse CD45.2
C57BL/6 mouse splenocytes were stained with CD45.2 (clone 10... -
Brilliant Violet 605™ anti-mouse CD45.2
C57BL/6 mouse splenocytes were stained with CD45.2 (clone 10... -
Purified anti-mouse CD45.2 (Maxpar® Ready)
C57BL/6 mouse bone marrow cells stained with 147Sm-anti-CD45... -
PE/Dazzle™ 594 anti-mouse CD45.2
C57BL/6 mouse splenocytes were stained with CD45.2 (clone 10... -
Brilliant Violet 711™ anti-mouse CD45.2
C57BL/6 mouse splenocytes were stained with CD45.2 (clone 10... -
Alexa Fluor® 594 anti-mouse CD45.2
BALB/C mouse frozen spleen section was fixed with 4% parafor... -
APC/Fire™ 750 anti-mouse CD45.2
C57BL/6 mouse splenocytes were stained with CD45.2 (clone 10... -
TotalSeq™-A0157 anti-mouse CD45.2
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TotalSeq™-B0157 anti-mouse CD45.2
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TotalSeq™-C0157 anti-mouse CD45.2
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Brilliant Violet 750™ anti-mouse CD45.2
C57BL/6 mouse splenocytes were stained with CD45.2 (clone 10... -
Spark Blue™ 550 anti-mouse CD45.2
C57BL/6 mouse splenocytes were stained with CD45.2 (clone 10... -
Spark NIR™ 685 anti-mouse CD45.2
C57BL/6 mouse splenocytes were stained with anti-mouse CD45.... -
Spark UV™ 387 anti-mouse CD45.2
C57BL/6 mouse splenocytes were stained with anti-mouse CD45.... -
Spark Blue™ 574 anti-mouse CD45.2 (Flexi-Fluor™)
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Spark Red™ 718 anti-mouse CD45.2 (Flexi-Fluor™)