TotalSeq™-C0215 anti-human CD268 (BAFF-R) Antibody

Pricing & Availability
Clone
11C1 (See other available formats)
Regulatory Status
RUO
Other Names
TNFRSF13C, BAFF-R, BAFFR, BR3, BAFF Receptor
Isotype
Mouse IgG1, κ
Barcode Sequence
CGAAGTCGATCCGTA
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Cat # Size Price Quantity Check Availability
316927 10 µg $369.00
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Description

B cell-activating factor receptor (BAFF-R) is a 19 kD type III membrane protein. It belongs to TNFR superfamily, also known as TNFRSF member 13C (TNFRSF13C), BAFF receptor 3 (BR3), or CD268. BAFF-R is expressed on mature B cells, B cell lymphoma, and T cell subset. BAFF-R is the major receptor for BAFF/BLys (or TALL-1, THANK) which binds to TACI and BCMA as well. The interaction of BAFF with BAFF-R promotes NF-κB activation and plays predominant roles in B-cell maturation and survival as well as costimulates T cell activation and proliferation. TRAF3 is a BAFF-R intracellularly associated protein, which negatively regulates BAFF-R-mediated NF-κB activation.

Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
BAFF-R-L1.2 transfectants
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA
Preparation
The antibody was purified by chromatography and conjugated with TotalSeq™-C oligomer under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

PG - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-C antibodies are compatible with 10x Genomics Chromium Single Cell Immune Profiling Solution.

To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions.


Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform.
Additional Product Notes

TotalSeq™ reagents are designed to profile protein levels at a single cell level following an optimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g. 10x Genomics Chromium System and Reagents) and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq.

The barcode flanking sequences are CGGAGATGTGTATAAGAGACAGNNNNNNNNNN (PCR handle), and NNNNNNNNNCCCATATAAGA*A*A (capture sequence). N represents either randomly selected A, C, G, or T, and * indicates a phosphorothioated bond, to prevent nuclease degradation.

View more applications data for this product in our Scientific Poster Library.

Application References

(PubMed link indicates BioLegend citation)
  1. Ng LG, et al.2004. J. Immunol. 173:807. (FC IHC)
  2. Personal communication. (Block)
RRID
AB_2814172 (BioLegend Cat. No. 316927)

Antigen Details

Structure
Type III transmembrane protein, TNF receptor superfamily, 19 kD
Distribution

Mature B cells, B cell lymphoma, T cell subset

Function
Induce B cell proliferation and immunoglobulin secretion, costimulate T cell activation
Ligand/Receptor
BAFF/BLys, associate with TRAF3
Cell Type
B cells, Leukemia, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Thompson JS, et al. 2001. Science 293:2108.
2. Ng LG, et al.2004. J. Immunol. 173:807.
3. Rodig SJ, et al. 2005. Human Pathol. 36:1113.
4. Ye Q, et al. 2004. Eur. J. Immunol. 34:2750.
5. Kayagaki N, et al. 2002 Immunity 17:515.

Gene ID
115650 View all products for this Gene ID
UniProt
View information about CD268 on UniProt.org
Go To Top Version: 1    Revision Date: 07/18/2019

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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