TotalSeq™-C0591 anti-human LOX-1 Antibody

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

LOX-1 ectodomain: Fc fusion protein

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA

Preparation

The antibody was purified by chromatography and conjugated with TotalSeq™-C oligomer under optimal conditions.

Concentration

0.5 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

PG - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-C antibodies are compatible with 10x Genomics Chromium Single Cell Immune Profiling Solution.

To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions.

Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform.

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescent staining of acetone-fixed frozen human skin sections¹.

Additional Product Notes

TotalSeq™ reagents are designed to profile protein levels at a single cell level following an optimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g. 10x Genomics Chromium System and Reagents) and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq.

The barcode flanking sequences are CGGAGATGTGTATAAGAGACAGNNNNNNNNNN (PCR handle), and NNNNNNNNNCCCATATAAGA*A*A (capture sequence). N represents either randomly selected A, C, G, or T, and * indicates a phosphorothioated bond, to prevent nuclease degradation.

View more applications data for this product in our Scientific Poster Library.

Application References

(PubMed link indicates BioLegend citation)

1. Li D, et al. 2012. J. Exp. Med. 209:109. (IHC)

RRID

AB_2832709 (BioLegend Cat. No. 358613)

Antigen Details

Structure

Single pass, type II, transmembrane protein, 31 kD, homodimer, member of the C-type lectin superfamily.

Distribution

Endothelial cells, smooth muscle, platelets, fibroblasts, macrophages; Upregulated by inflammatory and oxidative stimuli

Function

Scavenger receptor, involved in endocytosis, phagocytosis and cytokine production

Ligand/Receptor

Recognizes numerous ligands including lipoproteins, apoptotic cells, heat shock proteins, C-reactive protein, oxidized LDL

Cell Type

Endothelial cells, Platelets, Fibroblasts, Macrophages, Dendritic cells

Biology Area

Cell Adhesion, Cell Biology, Immunology, Innate Immunity

Molecular Family

Adhesion Molecules

Antigen References

1. Yin Y, et al. 2013. Eur. J. Cell Biol. 92:150. 
2. Khaidakov M. and Mehta JL. 2012. PLoS One. 7:e46973. 
3. Sawamura T, et al. 2012. Curr. Opin. Lipidol. 23:439. 
4. Lin FY, et al. 2011. J. Immunol. 186:4405. 
5. Parlato S, et al. 2010. Blood 115:1554.

Gene ID

4973 View all products for this Gene ID

UniProt

View information about LOX-1 on UniProt.org

Related Protocols

• TotalSeq™-B or -C with 10x Feature Barcoding Technology

Other Formats