TotalSeq™-C0862 anti-human CD218a (IL-18Rα) Antibody

Product Details

Verified Reactivity

Human, Cynomolgus, Rhesus

Reported Reactivity

African Green, Baboon

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Human NK cell line NK0 constitutively expressing IL-18 receptors

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA

Preparation

The antibody was purified by chromatography and conjugated with TotalSeq™-C oligomer under optimal conditions.

Concentration

0.5 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

PG - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-C antibodies are compatible with 10x Genomics Chromium Single Cell Immune Profiling Solution.

To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions.

Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform.

Application Notes

 The H44 antibody is specific for IL-18 receptor a chain. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections and neutralization¹.

Additional Product Notes

TotalSeq™ reagents are designed to profile protein levels at a single cell level following an optimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g. 10x Genomics Chromium System and Reagents) and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq.

The barcode flanking sequences are CGGAGATGTGTATAAGAGACAGNNNNNNNNNN (PCR handle), and NNNNNNNNNCCCATATAAGA*A*A (capture sequence). N represents either randomly selected A, C, G, or T, and * indicates a phosphorothioated bond, to prevent nuclease degradation.

View more applications data for this product in our Scientific Poster Library.

Application References

(PubMed link indicates BioLegend citation)

1. Kitasato Y, et al. 2004. Am. J. Respir. Cell Mol. Biol. 31:619. (IHC)
2. Vermot-Desroches C, et al. 2005. Cell Immunol. 236:101. (FC)

RRID

AB_2910384 (BioLegend Cat. No. 313817)

Antigen Details

Structure

Type I membrane protein, IL-1 receptor family, approximately 75-80 kD

Distribution

Expressed on NK cells and neutrophils, IL-12 activated T and B cells, endothelial cells, and smooth muscle cells; highly expressed on Hodgkin's disease cell lines

Function

Binds IL-18 with low affinity; high affinity binding site formed with IL-18Rβ

Ligand/Receptor

IL-18

Cell Type

B cells, Endothelial cells, Neutrophils, NK cells, T cells

Biology Area

Immunology, Innate Immunity

Molecular Family

CD Molecules, Cytokine/Chemokine Receptors

Antigen References

1. Torigoe K, et al. 1997. J. Biol. Chem. 272:25737.
2. Gerdes N, et al. 2002. J. Exp. Med. 195:245.
3. Airoldi I, et al. 2000. J. Immunol. 165:6880.

Gene ID

8809 View all products for this Gene ID

UniProt

View information about CD218a on UniProt.org

Related Protocols

• TotalSeq™-B or -C with 10x Feature Barcoding Technology

Other Formats