- Clone
- 1D12 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- HCDM listed
- Other Names
- T cell immunoglobulin mucin 1, TIMD-1, KIM-1 (kidney injury molecule-1), HAVCR-1, CD365
- Isotype
- Mouse IgG1, κ
- Barcode Sequence
- CTTCTGGGATTCTGG
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 353915 | 10 µg | $369.00 | |||
CD365 (Tim-1) is a transmembrane glycoprotein expressed on activated CD4+ lymphocytes especially on Th2 cells. It has been implicated to play a critical role in the development of atopic disease and other Th2-biased immune responses. Tim-1 is a hepatitis A virus receptor in humans. Tim-4 is the endogenous ligand of Tim-1. The interaction of Tim-1 and Tim-4 is involved in the costimulation of T cell proliferation. Tim-1 has been reported to be an endogenous ligand for LMIR5 (Leukocyte mono-immunoglobulin (Ig)-like receptor 5), and the Tim-1-LMIR5 interaction plays a physiological role in immune regulation by myeloid cells.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human TIM-1-IgV Fc
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA
- Preparation
- The antibody was purified by chromatography and conjugated with TotalSeq™-C oligomer under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
PG - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-C antibodies are compatible with 10x Genomics Chromium Single Cell Immune Profiling Solution.
To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions.
Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform. - Application Notes
-
Additional reported application (for the relevant formats) include: blocking function1.
- Additional Product Notes
-
TotalSeq™ reagents are designed to profile protein levels at a single cell level following an optimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g. 10x Genomics Chromium System and Reagents) and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq.
The barcode flanking sequences are CGGAGATGTGTATAAGAGACAGNNNNNNNNNN (PCR handle), and NNNNNNNNNCCCATATAAGA*A*A (capture sequence). N represents either randomly selected A, C, G, or T, and * indicates a phosphorothioated bond, to prevent nuclease degradation.
View more applications data for this product in our Scientific Poster Library. -
Application References
(PubMed link indicates BioLegend citation) -
- Freeman GJ, et al. 2007. Immunity. 27:927. (Block)
- RRID
-
AB_2890782 (BioLegend Cat. No. 353915)
Antigen Details
- Structure
- Type 1 transmembrane glycoprotein.
- Distribution
-
Activated CD4+ lymphocytes, especially on Th2 cells.
- Function
- TIM-1 ligation induces T cell proliferation, promotes cytokine production, and aids in the regulation of Th2 immunity.
- Ligand/Receptor
- TIM-4
- Cell Type
- Dendritic cells, T cells, Th2
- Biology Area
- Immunology, Inhibitory Molecules
- Molecular Family
- CD Molecules
- Antigen References
-
1. Freeman GJ, et al. 2007. Immunity. 27:927.
2. Meyers JH, et al. 2005. Nat. Immunol. 6:455.
3. Kuchroo VK, et al. 2003. Nat. Rev. Immunol. 3:454.
4. Mariat C, et al. 2005. Philos. Trans. R. Soc. Lond. B. 360:1681.
5. Yamanishi Y, et al. 2010. J. Exp. Med. 207:1501. - Gene ID
- 26762 View all products for this Gene ID
- UniProt
- View information about CD365 on UniProt.org
Other Formats
View All CD365 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human CD365 (Tim-1) | 1D12 | FC |
| PE anti-human CD365 (Tim-1) | 1D12 | FC |
| APC anti-human CD365 (Tim-1) | 1D12 | FC |
| PerCP/Cyanine5.5 anti-human CD365 (Tim-1) | 1D12 | FC |
| PE/Cyanine7 anti-human CD365 (Tim-1) | 1D12 | FC |
| TotalSeq™-C0869 anti-human CD365 (Tim-1) Antibody | 1D12 | PG |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD365 (Tim-1)
769-P, human kidney cell line was stained with purified anti... -
PE anti-human CD365 (Tim-1)
769-P, human kidney cell line was stained with anti-human CD... -
APC anti-human CD365 (Tim-1)
Human kidney cell line 769-P was stained with anti-human CD3... -
PerCP/Cyanine5.5 anti-human CD365 (Tim-1)
769-P, human kidney cell line was stained with anti-human CD... -
PE/Cyanine7 anti-human CD365 (Tim-1)
769-P, human kidney cell line was stained with anti-human CD... -
TotalSeq™-C0869 anti-human CD365 (Tim-1) Antibody