- Clone
- HMD1-3 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Dll 1, Dll-1
- Isotype
- Armenian Hamster IgG
- Barcode Sequence
- AGACCTCCTTACGAT
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 128317 | 10 µg | $369.00 | |||
The Notch receptors and their ligands are highly conserved from invertebrates to mammals. The Delta-like 1 (DLL1) is one of the four or five Notch ligands identified. The binding to Notch receptor results in the proteolysis of Notch and movement of intracellular portion of Notch into the nucleus.This translocation triggers a series of signaling process. Delta-like 1 is reported to be essential for the maintenance of marginal zone B cells in normal mice, and engagement of Notch 1 by DLL1 promotes differentiation of B lymphocytes to antibody-secreting cells.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- CHO cells expressing murine Dll 1
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA
- Preparation
- The antibody was purified by chromatography and conjugated with TotalSeq™-C oligomer under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
PG - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-C antibodies are compatible with 10x Genomics Chromium Single Cell Immune Profiling Solution.
To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions.
Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform. - Additional Product Notes
-
TotalSeq™ reagents are designed to profile protein levels at a single cell level following an optimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g. 10x Genomics Chromium System and Reagents) and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq.
The barcode flanking sequences are CGGAGATGTGTATAAGAGACAGNNNNNNNNNN (PCR handle), and NNNNNNNNNCCCATATAAGA*A*A (capture sequence). N represents either randomly selected A, C, G, or T, and * indicates a phosphorothioated bond, to prevent nuclease degradation.
View more applications data for this product in our Scientific Poster Library. - RRID
-
AB_2888794 (BioLegend Cat. No. 128317)
Antigen Details
- Structure
- A single pass transmembrane protein consisting of a large extracellular N-terminal which includes the DSL domain, followed by a tandem array of EGF-like repeats (ELRs). After a single transmembrane domain (TM) is a novel intracellular domain.
- Distribution
-
B cells
- Function
- The Notch receptors and their ligands are highly conserved signaling molecules throughout development. They regulate cell fate decision for various cell development, including myogenesis, neurogenesis, and lymphocyte development.
- Ligand/Receptor
- Notch receptors
- Cell Type
- B cells
- Biology Area
- Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
- Molecular Family
- Adhesion Molecules
- Antigen References
-
1. Ehebauer MT, et al. 2006. Biochem. J. 392:13.
2. Shimizu K, et al. 2000. Mol. Cell. Biology 20:18.
3. Parks AL, et al.2006. Genetics 174:1947.
4. Santos MA, et al. 2007. P. Natl. Acad. Sci. USA 104:15454. - Gene ID
- 13388 View all products for this Gene ID
- UniProt
- View information about DLL1 on UniProt.org
Other Formats
View All DLL1 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-mouse DLL1 | HMD1-3 | FC |
| PE anti-mouse DLL1 | HMD1-3 | FC |
| APC anti-mouse DLL1 | HMD1-3 | FC |
| TotalSeq™-C0884 anti-mouse DLL1 Antibody | HMD1-3 | PG |
| TotalSeq™-B0884 anti-mouse DLL1 Antibody | HMD1-3 | PG |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse DLL1
FD1/CHO (mouse DLL1 transfected cel l line) stained with pur... -
PE anti-mouse DLL1
LPS-stimulated BALB/c peritoneal macrophages (Day 2) stained... -
APC anti-mouse DLL1
FD1/CHO (mouse DLL1) transfected cell line stained with HMD1... -
TotalSeq™-C0884 anti-mouse DLL1 Antibody
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TotalSeq™-B0884 anti-mouse DLL1 Antibody