- Clone
- TH6 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Folate receptor 4, FBP3, FR δ
- Isotype
- Rat IgG2b, κ
- Barcode Sequence
- TGATCTAAGGTATGC
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 125113 | 10 µg | $369.00 | |||
Folate receptor 4 (FR4) is a surface receptor for folic acid (Vitamin B9).It was recently reported that FR4 is highly and constitutively expressed on mouse CD4+ CD25+ natural regulatory T cells (Treg).The high expression of this surface marker on Treg can be used in combination with CD4 and CD25 to distinguish Treg from other types of T cells.Since FR4 is a surface marker,this antibody may be used in combination with anti-CD4 and/or CD25 to sort living T reg cells for functional studies.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA
- Preparation
- The antibody was purified by chromatography and conjugated with TotalSeq™-C oligomer under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
PG - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-C antibodies are compatible with 10x Genomics Chromium Single Cell Immune Profiling Solution.
To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions.
Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform. - Additional Product Notes
-
TotalSeq™ reagents are designed to profile protein levels at a single cell level following an optimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g. 10x Genomics Chromium System and Reagents) and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq.
The barcode flanking sequences are CGGAGATGTGTATAAGAGACAGNNNNNNNNNN (PCR handle), and NNNNNNNNNCCCATATAAGA*A*A (capture sequence). N represents either randomly selected A, C, G, or T, and * indicates a phosphorothioated bond, to prevent nuclease degradation.
View more applications data for this product in our Scientific Poster Library. -
Application References
(PubMed link indicates BioLegend citation) -
1. Yamaguchi T et al. 1007: Immunity. 27(1)145
2. Inoue N, et al. 2015. Nat Commun. 6: 8858. PubMed
3. Kato K, et al. 2016. Nat Commun. 7:12198. PubMed - RRID
-
AB_2876448 (BioLegend Cat. No. 125113)
Antigen Details
- Structure
- 28 kd protein consisting of 244 amino acids. Heavily glycosylated
- Distribution
-
Expressed on mouse thymocytes and spleen cells. Highly expressed on natural regulatory (Treg) cells
- Function
- Mediate calcium-independent homophlic cell-cell adhesion
- Ligand/Receptor
- Folic acid and its derivative
- Cell Type
- Thymocytes, Tregs
- Biology Area
- Immunology, Innate Immunity
- Antigen References
-
1. Yamaguchi T, et al. 2007. Immunity. 27:145
2. Spiegelstein O, et al. 2000. Gene. 258:117 - Gene ID
- 64931 View all products for this Gene ID
- UniProt
- View information about FR4 on UniProt.org
Other Formats
View All Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Biotin anti-mouse FR4 (Folate Receptor 4) | TH6 | FC |
| PE anti-mouse FR4 (Folate Receptor 4) | TH6 | FC |
| TotalSeq™-C1058 anti-mouse FR4 (Folate Receptor 4) | TH6 | PG |
| Ultra-LEAF™ Purified anti-mouse FR4 (Folate Receptor 4) Antibody | TH6 | FC,Cyt,Depletion |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Biotin anti-mouse FR4 (Folate Receptor 4)
BALB/c mouse splenocytes stained with CD3 (145-2C11) APC and... -
PE anti-mouse FR4 (Folate Receptor 4)
BALB/c mouse splenocytes stained with TH6 PE and CD3 (145-2C... -
TotalSeq™-C1058 anti-mouse FR4 (Folate Receptor 4)
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Ultra-LEAF™ Purified anti-mouse FR4 (Folate Receptor 4) Antibody
C57BL/6 mouse splenocytes stained with CD3 (145-2C11) APC an...