TotalSeq™-B0016 anti-human Galectin-9 Antibody

Pricing & Availability
Clone
9M1-3 (See other available formats)
Regulatory Status
RUO
Other Names
Lgals9, Gal-9, Ecalectin, Tumor antigen HOM-HD-21, Ecalectin
Isotype
Mouse IgG1, κ
Barcode Sequence
ACTCACTGGAGTCTC
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Cat # Size Price Quantity Check Availability
348927 10 µg $369.00
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Description

Galectin-9 is a mammalian lectin with a molecular weight around 50 kD. It is a member of the β-galactoside-binding family. With two conserved carbohydrate recognition domains (CRDs), galectin-9 binds small β-galactosides as well as complex glycoconjugates.  HAVCR2/TIM3 has been reported as one of its ligands. Galectin-9 may be retained intracellularly or transported to the cell surface where it can be cleaved to generate a soluble form. Galectin-9 is expressed by lymphocytes, dendritic cells, granulocytes, eosinophils, astrocytes, endothelial cells, fibroblasts, and thymus epithelial cells. It can be induced by cytokines in various cell types and is involved in cell aggregation, adhesion, chemotaxis, and apoptosis; galectin-9 induces regulatory T cells and suppresses Th1 and Th17 responses.

Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant peptide from C-terminus of Galectin-9
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA
Preparation
The antibody was purified by chromatography and conjugated with TotalSeq™-B oligomer under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ICPG - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-B antibodies are compatible with 10x Genomics Single Cell Gene Expression Solutions.

To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 0.5 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions.


Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform.
Application Notes

Additional reported applications (for the relevant formats) include: cell surface staining for flow cytometry1 and blocking of TIM-3 binding to galectin-91.

Additional Product Notes

TotalSeq™ reagents are designed to profile protein levels at a single cell level following an optimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g. 10x Genomics Chromium System and Reagents) and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq.

The barcode flanking sequences are GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTNNNNNNNNNN (PCR handle), and NNNNNNNNNGCTTTAAGGCCGGTCCTAGC*A*A (capture sequence). N represents either randomly selected A, C, G, or T, and * indicates a phosphorothioated bond, to prevent nuclease degradation.

View more applications data for this product in our Scientific Poster Library.

Application References

(PubMed link indicates BioLegend citation)
  1. Klibi J, et al. 2009. Blood 113:1957. (FC, Block)
  2. Sada-Ovalle I, et al. 2012. J. Immunol. 189:5896. PubMed
RRID
AB_3106227 (BioLegend Cat. No. 348927)

Antigen Details

Structure
Member of the β-galactoside-binding lectin family. Molecular weight around 50 kD. Binds small β-galactosides as well as complex glycoconjugates with two conserved carbohydrate recognition domains (CRDs).
Distribution

Lymphocytes, dendritic cells, neutrophils, eosinophils, astrocytes, endothelial cells, fibroblasts, thymus stromal/epithelial cells.

Function
Cell aggregation, adhesion, chemotaxis, apoptosis, suppression of Th1 and Th17 responses, and induction of regulatory T cells.
Ligand/Receptor
HAVCR2/TIM3
Cell Type
Astrocytes, Dendritic cells, Endothelial cells, Eosinophils, Epithelial cells, Fibroblasts, Lymphocytes, Neutrophils, Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology
Molecular Family
Adhesion Molecules, Immune Checkpoint Receptors
Antigen References

1. Seki M, et al. 2008. Clin. Immunol. 127:78.
2. Tsuboi Y, et al. 2007. Clin. Immunol. 124:221.
3. Zhu C, et al. 2005. Nat. Immunol. 6:1245.
4. Dunphy JL, et al. 2002. J. Biol. Chem. 277:14916.

Gene ID
3965 View all products for this Gene ID
UniProt
View information about Galectin-9 on UniProt.org
Go To Top Version: 1    Revision Date: 05/28/2024

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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