- Clone
- W22113E (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Herpes virus entry mediator (HVEM), TNFRSF14, ATAR, CD270, Herpesvirus entry mediator A, Tumor necrosis factor receptor superfamily, member 14,
- Isotype
- Rat IgG2b, κ
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Biotinylated recombinant human BTLA-Fc chimera (Cat. No. 794906) (black circles) binds to immobilized human HVEM-Fc chimera (Cat. No. 596506). Ultra-LEAF™ purified anti-human CD270 (HVEM) (clone W22113E) (purple squares) inhibits the binding in a dose-dependent manner, whereas the Ultra-LEAF™ purified rat IgG2b, κ isotype control (Cat. No. 400644) (gray triangles) does not have this effect. This antibody blocks the binding of human BTLA-Fc chimera to 0.1 µg/mL immobilized recombinant human HVEM-Fc chimera with ND50 range: 18 – 110 ng/mL.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 636153 | 100 µg | $420.00 | |||
| 636154 | 1 mg | $1065.00 | |||
Herpes virus entry mediator (HVEM), a member of the tumor necrosis factor (TNF) receptor family, mediates herpes virus entry into cells during infection. Upon overexpression, HVEM activates NF-κB and AP-1 through a TNF receptor-associated factor (TRAF)-mediated mechanism. HVEM ligands belong to two distinct families: the TNF-related cytokines LIGHT and lymphotoxin-α, and the Ig-related membrane proteins BTLA and CD160. HVEM and its ligands have been involved in the pathogenesis of various autoimmune and inflammatory diseases, tumor progression and resistance to immune response. It has been demonstrated that HVEM induces a novel signaling pathway downstream leading to signal transduction and activation of STAT3 in epithelial cells. Since STAT3 regulates the expression of genes important for host defense in epithelial cells, as well as the differentiation of retinoid-related orphan receptor RORγt+, Th17, and innate lymphoid cells. This finding suggests that HVEM may play an important role in mucosal host defense.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant Human HVEM
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
Block - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by blocking the binding of 0.8 μg/mL Recombinant Human BTLA-Fc Chimera to 0.1 μg/mL immobilized human HVEM-Fc Chimera. ND50 range: 18 – 110 ng/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
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AB_3662317 (BioLegend Cat. No. 636153)
AB_3662317 (BioLegend Cat. No. 636154)
Antigen Details
- Distribution
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Spleen, thymus, lung, macrophages, B cells and T cells
- Function
- HVEM plays a role in mucosal host defense, autoimmune and inflammatory diseases, and tumor progression
- Interaction
- T cells, NK cells
- Ligand/Receptor
- LIGHT, lymphotoxin-alpha, BTLA, CD160, TRAF proteins
- Cell Type
- Tregs
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Signal Transduction
- Molecular Family
- Adhesion Molecules, CD Molecules, Immune Checkpoint Receptors
- Antigen References
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- Pasero C, et al. 2012. Curr Opin Pharmacol. 12:478-85.
- Hsu H, et al. 1997. J Biol Chem. 272:13471-4.
- Mauri DN, et al. 1998. Immunity. 8:21-30.
- Montgomery RI, et al. 1996. Cell. 87:427-36.
- Shui JW, et al. 2012. Nature. 488:222-5.
- Gene ID
- 8764 View all products for this Gene ID
- UniProt
- View information about CD270 on UniProt.org
Other Formats
View All CD270 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Ultra-LEAF™ Purified anti-human CD270 (HVEM) | W22113E | Block |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Ultra-LEAF™ Purified anti-human CD270 (HVEM)
Biotinylated recombinant human BTLA-Fc chimera (Cat. No. 794...