- Clone
- W20208A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- ALRH, BHR1, P600, IL-13, MGC116786, MGC116788, MGC116789, IL13
- Isotype
- Rat IgG2b, κ
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Recombinant human IL-13 (Cat. No. 571106) (black circles) induces proliferation of human TF-1 cells. Ultra-LEAF™ purified anti-human IL-13 (clone W20208A) (purple squares) neutralizes the TF-1 cell proliferation induced by recombinant human IL-13 at 15 ng/mL in a dose-dependent manner, whereas the GoInVivo™ purified rat IgG2b, κ isotype control (Cat. No. 400666) (gray triangles) does not have the effect. ND50 range: 0.015 - 0.08 µg/mL.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 604153 | 100 µg | $231.00 | |||
| 604154 | 1 mg | $1205.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
Human IL-13 was initially cloned from cDNA libraries of activated T cells. IL-13 is an immunoregulatory cytokine secreted predominantly by activated T(H)2 cells, and it is a key mediator in the pathogenesis of allergic inflammation. IL-13 shares many functional properties with IL-4, and they share a common receptor subunit, the alpha subunit of the IL-4 receptor (IL-4Ralpha). IL-13 mediates its effects by interacting with a complex receptor system comprised of IL-4Ralpha and two IL-13 binding proteins, IL-13Ralpha1 and IL-13Ralpha2. Ligation of the IL-13 receptor complex results in signaling via the insulin receptor substrate (IRS)-1 and 2 and STAT-6 pathways. IL-13, like IL-4, is a cytokine produced by T(H)2 type helper T cells in response to signaling through the T cell antigen receptor and by mast cells and basophils upon cross-linkage of the high-affinity receptor for immunoglobulin E (IgE). IL-13 has been implicated in airway hypersensitivity and mucus hypersecretion, inflammatory bowel disease, and parasitic nematode expulsion.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant human IL-13
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
Neut - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by the inhibition of proliferation induced by recombinant human IL-13 (Cat. No. 571106) at 15 ng/mL on TF-1 cells. ND50 range: 0.015 - 0.08 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2936677 (BioLegend Cat. No. 604153)
AB_2936677 (BioLegend Cat. No. 604154)
Antigen Details
- Structure
- Cytokine
- Distribution
-
IL-13 is secreted by activated T cells, Th2 cells, basophils, mast cells, activated eosinophils and NK cells.
- Function
- IL-13 modulates human monocytes/macrophages and B cells. IL-13 induces an increase of MHC class II expression in human monocytes/macrophages, and direct inhibition of inflammatory cytokines such as TNF-α and IL-1β
- Interaction
- IL-13 receptors are expressed on human B cells, basophils, eosinophils, mast cells, endothelial cells, fibroblasts, monocytes, macrophages, respiratory epithelial cells, and smooth muscle cells.
- Ligand/Receptor
- IL-4Ralpha, IL-13Ralpha1, and IL-13Ralpha2
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
- McKenzie AN, et al. 1993. Proc Natl Acad Sci U S A. 90:3735-9.
- Papasavvas E, et al. 2005. J Immunol. 175:5532-40.
- Kelly-Wellch A, et al. 2005. Sci STKE. 2932005.
- Hershey GK. 2003. J Allergy Clin Immunol. 111:677-90.
- Harris J, et al. 2007. Immunity. 27:505-17.
- LaPorte SL, et al. 2008. Cell. 132:259-72.
- Gene ID
- 3596 View all products for this Gene ID
- UniProt
- View information about IL-13 on UniProt.org
Other Formats
View All IL-13 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Ultra-LEAF™ Purified anti-human IL-13 | W20208A | Neut |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Ultra-LEAF™ Purified anti-human IL-13
Recombinant human IL-13 (Cat. No. 571106) (black circles) in...