Ultra-LEAF™ Purified anti-Asialo-GM1 Antibody

Pricing & Availability
Clone
Poly21460 (See other available formats)
Regulatory Status
RUO
Other Names
Ganglioside, GM1, ganglio-N-tetraosylceramide
Isotype
Rabbit Polyclonal IgG
Poly21460_ULEAF_Asialo-GM1_Antibody_1_101524
C57BL/6 mouse splenocytes were stained with Ultra-LEAF™ purified anti-Asialo-GM1 (clone Poly21460), followed by anti-rabbit IgG FITC and NK1.1 PE/Cyanine7.
  • Poly21460_ULEAF_Asialo-GM1_Antibody_1_101524
    C57BL/6 mouse splenocytes were stained with Ultra-LEAF™ purified anti-Asialo-GM1 (clone Poly21460), followed by anti-rabbit IgG FITC and NK1.1 PE/Cyanine7.
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Cat # Size Price Quantity Check Availability
146002 1 mL $534.00
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Description

GM1 is a ganglioside, a type of glycosphingolipid with a single sialic acid group. Asialo-GM1 is a GM1 derivative without a sialic acid group. It is expressed on NK cells, basophils, monocytes/macrophages, and T cells. It is particularly expressed on very early thymocytes, but the expression decreases as the cells mature and become Thy-1+. The highest expression is detected on neuronal tissues. This molecule has been shown to be involved in microbial pathogenesis. Antibodies specific for Asialo-GM1 are elevated in dementia, lupus, and Guillain-Barré syndrome.

Technical data sheet

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Asialo-GM1
Formulation
Lyophilized powder.
Preparation
Reconstitute with 1 ml sterile deionized, distilled, endotoxin-free water. Gamma globulin fraction of serum was precipitated using 50% ammonium sulfate followed by dialysis with phosphate buffered saline.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
Depletion - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Optimal staining for flow cytometry may vary from lot-to-lot. This antibody has been reported to deplete NK cells in vivo. A usage of 50 µl per mouse is recommended, but optimal usage will vary depending on the strain of mouse and experimental conditions2-4. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody recognizes asialo-GM1. It does not react with other glycolipids, such as GM1 and Asialo-GM2.

This antibody can partially block IL-12 induced IFN-γ production but does not affect other systemic action of IL-12. 

This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Naiki M, et al. 1974. J. Immunol. 113:84.
  2. Kasai M, et al. 1981. Nature. 291:334. (Depletion)
  3. Nishikado H, et al. 2011. J. Immunol. 186:5766. (Depletion)
  4. Zhou G, et al. 2013. Eur. J. Immunol. 43:929. (Depletion)
Product Citations
  1. Wang X, et al. 2022. Viruses. 14:. PubMed
  2. Böttcher JP, et al. 2018. Cell. 172:1022. PubMed
  3. Hu J, et al. 2015. J Virol. 89: 10383 - 10398. PubMed
  4. Li P, et al. 2020. Nat Commun. 3.504861111. PubMed
  5. André P et al. 2018. Cell. 175(7):1731-1743 . PubMed
  6. Lin Q, et al. 2021. Nat Commun. 12:2537. PubMed
  7. Shannon JP, et al. 2021. Immunity. 54(2):276-290.e5. PubMed
  8. Ren Y, et al. 2015. Sci Rep. 5: 18099. PubMed
  9. Turaj AH et al. 2017. Cancer cell. 32(6):777-791 . PubMed
  10. Dai HS et al. 2017. Immunity. 47(1):159-170 . PubMed
  11. Gauthier L, et al. 2019. Cell. 177:1701. PubMed
  12. Pelly VS, et al. 2021. Cancer Discov. 11:2602. PubMed
  13. Manangeeswaran M, et al. 2020. Front Immunol. 11:237. PubMed
  14. Ma Q, et al. 2021. Precis Clin Med. 4:155. PubMed
  15. Bonavita E, et al. 2020. Immunity. 1215:53. PubMed
  16. Dubrot J, et al. 2021. Immunity. 54(3):571-585.e6. PubMed
RRID
SCR_001134 (BioLegend Cat. No. 146002)

Antigen Details

Structure
Asialo-GM1 is a GM1 derivative without a sialic acid group, with the structure: βDGalp(1-3)βDGalpNAc(1-4)βDGalp(1-4)βDGlcp(1-1)Cer
Distribution

NK cells, basophils, splenic T lymphocytes, highest concentrations on neurons

Function
Supports cellular identification, neuronal plasticity, and repair mechanisms
Interaction
Facilitates cellular recognition and cell-to-cell communication
Ligand/Receptor
Ligand for cholera toxin
Cell Type
Basophils, Neurons, NK cells, T cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction
Antigen References

1. Stein-Douglas K, et al. 1979. J. Exp. Med. 143:822. 
2. Kasai M, et al. 1980. Eur. J. Immunol. 10:175. 
3. Young WW Jr, et al. 1980. J. Immunol. 124:199. 
4. Bansal AS, et al. 1994. J. Clin. Path. 47:300.

Gene ID
NA
UniProt
View information about Asialo-GM1 on UniProt.org
Go To Top Version: 5    Revision Date: 10/06/2022

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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