- Clone
- L403H11 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Eotaxin, eotaxin-1, small inducible cytokine subfamily A, member 11 (SCYA11)
- Isotype
- Rat IgG1, κ
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Ultra-LEAF™ Purified anti-mouse CCL11 Antibody inhibits the chemotaxis induced by mouse CCL11 on mouse CCR3 transfected Baf3 mouse cells in a dose dependent manner, with an ED50= 0.25 - 1.25 µg/mL. -
Ultra-LEAF™ Purified anti-mouse CCL11 Antibody inhibits the chemotaxis induced by mouse CCL11 on mouse CCR3 transfected Baf3 mouse cells in a dose dependent manner, with an ED50= 0.25 - 1.25 µg/mL.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 683203 | 500 µg | $545.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CCL11 is a member of the CC chemokine family and was originally identified as a chemoattractant for eosinophils. It participates in a variety of allergic diseases in response to allergens. CCL11 induces angiogenic response by CCR3-positive microvascular endothelial cells. Also, CCL11 facilitates proliferation and metastasis of tumor cells expressing CCR3.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant Mouse CCL11, amino acids (His24-Pro97) (Accession# NM_011330), was expressed in E. coli.
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
Block - Quality tested
ELISA Capture, Neut - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by blocking chemotaxis induced by mouse CCL11 on mouse CCR3 transfected BA/F3 mouse cells. The ED50 is 0.25 - 1.25 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
For highly sensitive assays, we recommend Ultra-Leaf™ purified antibody (Cat. No. 683203) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
- RRID
-
AB_2571917 (BioLegend Cat. No. 683203)
Antigen Details
- Structure
- Chemokine.
- Distribution
-
Epithelial cells, smooth muscle cells, dendritic cells, fibroblasts, mast cells, and Th2 lymphocytes.
- Function
- Recruits eosinophils in allergic responses. Stimulates eosinophil release from bone marrow, increasing levels of circulating eosinophils. Induced by pro-inflammatory cytokines (IL-4, IL-13, TNF-7α, and IFN-α).
- Interaction
- Eosinophils.
- Ligand/Receptor
- CCR3.
- Cell Type
- Dendritic cells, Epithelial cells, Fibroblasts, Mast cells, Th2
- Biology Area
- Angiogenesis, Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Crump MP, et al. 1998. J. Biol. Chem. 273:22471.
2. Conroy DM, et al. 2001. Respir. Res. 2:150.
3. Salcedo R, et al. 2001. J. Immunol. 166:7571.
4. Hadjicharalambous C, et al. 2004. J. Allergy Clin. Immunol. 113:657.
5. Kaehler J, et al. 2006. J. Investig. Med. 54:446.
6. Levina V, et al. 2009. Clin. Cancer Res. 15:2647. - Gene ID
- 20292 View all products for this Gene ID
- UniProt
- View information about CCL11 on UniProt.org
Other Formats
View All CCL11 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Ultra-LEAF™ Purified anti-CCL11 | L403H11 | Block,ELISA Capture,Neut |
| Purified anti-CCL11 | L403H11 | ELISA Capture |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Ultra-LEAF™ Purified anti-CCL11
Ultra-LEAF™ Purified anti-mouse CCL11 Antibody inhibit... 
Ultra-LEAF™ Purified anti-mouse CCL11 Antibody inhibit... -
Purified anti-CCL11