- Clone
- 13A9 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CDHN, CDw325, NCAD, N-cadherin 1, Cadherin 2, N-cadherin (neuronal), cadherin-2, calcium-dependent adhesion protein, neuronal, neural cadherin, Neural-cadherin
- Isotype
- Mouse IgG1, κ
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Western blot of Ultra-LEAF™ Purified anti-CD325 (N-Cadherin) antibody (clone 13A9). Lane 1: Molecular weight marker; Lane 2: 10 µg of human brain membrane lysate; Lane 3: 10 µg of mouse brain membrane lysate; Lane 4: 10 µg of rat brain membrane lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 844703 | 100 µg | $160.00 | |||
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CD325 (N-cadherin) is important for neuronal migration during development, neurite outgrowth and target finding during differentiation, initial formation of synapses, and synaptic plasticity in mature neurons. CD325 (N-cadherin) is a 130 kD single pass transmembrane protein. Its extracellular region consists of five extracellular cadherin domains and has one cytoplasmic domain. N-cadherin is involved in organogenesis and in the maintenance of organ architecture by contributing to the sorting of heterogeneous cell types and in the cell adhesion needed to form tissues. N-cadherin is expressed by stem cells, myeloblasts, endothelial cells, fibroblasts, and also is expressed in neural and muscle tissues along with some types of carcinoma cells. CD325 associates with the cytoskeleton trough catenin proteins.
Product Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human N-cadherin cytoplasmic domain fused to recombinant MBP.
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunfluorescence microscopy1,2,3,6, immunohistochemical staining4, and immunoprecipitation2,5,7.
For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 844703) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg). -
Application References
(PubMed link indicates BioLegend citation) -
- Wahl JK, et al. 2003. J. Biol. Chem. 278:17269. (WB, IF)
- Knudsen KA, et al. 1995. J. Cell Biol. 130:67. (WB, IF, IP)
- Machell NH, et al. 2000. Biol. Reprod. 63:797. (WB, IF)
- Han AC, et al. 1997. Hum. Pathol. 28:641. (IHC)
- Van Aken EH, et al. 2002. Invest Ophthalmol. Vis. Sci. 43:595. (WB, IP)
- Tian G, et al. 2009. J. Biol. Chem. 284:18980. (IF)
- Nieman MT. 1999. J. Cell Biol. 147:631. (IP, WB)
- RRID
-
AB_2565807 (BioLegend Cat. No. 844703)
Antigen Details
- Structure
- Member of cadherin family, single pass transmembrane protein, extracellular region consists of five extracellular cadherin domains, one cytoplasmic domain, 130 kD.
- Distribution
-
Cell membrane, stem cells, myeloblasts, endothelial cells, fibroblasts, neural and muscle tissues, and some types of carcinoma cells.
- Function
- Embryonic development, maintain tissue architecture, cell sorting and cell adhesion in tissues, promote motility, cell junction organization, pre and post synaptic adhesion, and ERK signaling.
- Interaction
- Catenins.
- Cell Type
- Mesenchymal Stem Cells, Neural Stem Cells
- Biology Area
- Cell Biology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Stem Cells, Synaptic Biology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Hirano S, et al. 2012. Physiol. Rev. 92:597.
- Gene ID
- 1000 View all products for this Gene ID
- UniProt
- View information about CD325 on UniProt.org
Other Formats
View All CD325 (N-Cadherin) Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Ultra-LEAF™ Purified anti-CD325 (N-Cadherin) | 13A9 | WB |
| Purified anti-CD325 (N-Cadherin) | 13A9 | WB,ICC,IHC-P,IP |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Ultra-LEAF™ Purified anti-CD325 (N-Cadherin)
Western blot of Ultra-LEAF™ Purified anti-CD325 (N-Cadherin)... -
Purified anti-CD325 (N-Cadherin)
Western blot of purified anti-CD325 (N-Cadherin) antibody (c...