- Clone
- 8A7-G10 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Chromosome 14 Open Reading Frame 169, ROX, hsNO66, NO66, Nucleolar Protein 66, Ribosomal Oxygenase NO66, MAPJD
- Isotype
- Mouse IgG1, κ
-
70 µg of whole cell protein were separated by SDS-PAGE, transferred to a nitrocellulose membrane, and probed with αNO66 (clone 8A7-G10) diluted 1:100. The exposure time was 30 seconds.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 844903 | 100 µg | $380.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
Histone Lysine Demethylase (NO66) is an oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. It specfically demethylates 'Lys-4' (H3K4me) and 'Lys-36' (H3K36me) of histone H3, thereby playing a central role in the histone code.
Product Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Fusion protein consisting of NO66 protein fragment (aa 423-427) and Hepatitis B virus capsid protein.
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2566003 (BioLegend Cat. No. 844903)
Antigen Details
- Structure
- 641 amino acids; 71 kD.
- Distribution
-
Nucleus, nucleolus, and nucleoplasm.
- Function
- Preferentially demethylates trimethylated H3 'Lys-4' (H3K4me3) and momomethylated H3 'Lys-4' (H3K4me1) residues, while it has weaker activity for dimethylated H3 'Lys-36' (H3K36me2). Also catalyzes the hydroxylation of 60S ribosomal protein L8 on 'His-216' and acts as a regulator of osteoblast differentiation via its interaction with SP7/OSX by demethylating H3K4me and H3K36me, thereby inhibiting SP7/OSX-mediated promoter activation (by similarity). May also play a role in ribosome biogenesis and in the replication or remodeling of certain heterochromatic region. Participates in MYC-induced transcriptional activation.
- Biology Area
- Cell Biology, Neuroscience
- Antigen References
-
1. Vasilatos S, et al. 2013. Carcinogenesis 34:1196.
2. Iwamori N, et al. 2011. Biol. Reprod. 84:1225.
3. Tao Y, et al. 2013. J. Biol. Chem. 288:16430. - Gene ID
- 79697 View all products for this Gene ID
- UniProt
- View information about Histone on UniProt.org
Other Formats
View All Histone Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Histone Lysine Demethylase (NO66) | 8A7-G10 | WB |
| Ultra-LEAF™ Purified anti-Histone Lysine Demethylase (NO66) | 8A7-G10 | WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Histone Lysine Demethylase (NO66)
70 µg of whole cell protein were separated by SDS-PAGE, tran... -
Ultra-LEAF™ Purified anti-Histone Lysine Demethylase (NO66)
70 µg of whole cell protein were separated by SDS-PAGE, tran...