- Clone
- A20139D (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Bone morphogenetic protein 6, VGR1
- Isotype
- Mouse IgG2a, κ
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Recombinant human BMP-6 (Cat. No. 595502) (black circles) induces the differentiation of ATDC5 mouse chondrogenic cells. Ultra-LEAF™ purified anti-humam BMP-6 (clone A20139D) (purple squares) inhibits the effect of recombinant human BMP-6 at 0.1 µg/mL in ATDC5 cells in a dose-dependent manner, whereas the Ultra-LEAF™ purified mouse IgG2a, κ isotype control (Cat. No. 401507) (gray triangles) does not have effect. ND50 range: 0.1 - 0.6 µg/mL.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 643653 | 100 µg | $430.00 | |||
| 643654 | 1 mg | $1135.00 | |||
Bone morphogenetic proteins (BMPs) belong to the transforming growth factor beta (TGF-β) superfamily. BMPs play a key role in embryonic development, especially during heart, neural, and cartilage development. Around 20 BMP family members have been identified and characterized. BMPs signal through serine/threonine kinase receptors, composed of type I and II subtypes. Four type I receptors have been identified: type IA and IB BMP receptors, type IA activin receptor, and activin receptor-like kinase I (ALK-1). Three type II receptors have also been recognized: type II BMP receptor and type II and IIB activin receptors. Human recombinant BMP-6 can restore bone in animal models of osteoporosis. BMP-6 induces bone formation through IGF-I and EGF pathways in a murine model of osteopenia and in human osteoblasts. BMP-6 has been reported to have an antiproliferative effect on B, T cells, and macrophages. Also, BMP-6 induced expression of pro-inflammatory inducible nitric oxide synthase (iNOS), TNF-α and IL-1β in macrophages. In addition, BMP-6 is a key endogenous regulator of hepcidin expression and iron metabolism in vivo. In fact, BMP-6 null mice have a phenotype that resembles mouse models of juvenile hemochromatosis (an iron overload disorder caused by mutations in the genes encoding the major iron regulatory hormone hepcidin and hemojuvelin).
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant Human BMP-6
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
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Neut - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by neutralizing the differentiation induced by recombinant human BMP-6 (Cat. No. 595502) at 0.1 µg/mL on ATDC5 mouse chondrogenic cells. ND50 range: 0.1 - 0.6 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
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AB_3662325 (BioLegend Cat. No. 643653)
AB_3662325 (BioLegend Cat. No. 643654)
Antigen Details
- Structure
- Growth factor, Homodimer
- Distribution
-
Hepatocytes
- Function
- Osteoblast differentiation, bone regeneration. Antiproliferative effect on B, T cells, and macrophages. BMP-6 regulates hepcidin expression and iron metabolism in vivo.
- Interaction
- Cancer cells, mesenchymal stem cells germline stem cells, hematopoietic stem cells, and neural stem cells
- Ligand/Receptor
- BMP receptors Alk-2 and Alk-3 (type I), BMPRII (type II)
- Cell Type
- Embryonic Stem Cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Neural Stem Cells
- Biology Area
- Angiogenesis, Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Antigen References
-
- Chen D, et al. 2004. Growth Factors. 22:233-41.
- Grasser WA, et al. 2007. Int Orthop. 31:759-65.
- Sivertsen EA, et al. 2007. Eur J Immunol. 37:2937-48.
- Andriopoulos B, et al. 2009. Nat Genetics. 41:482-7.
- Hong JH, et al 2009. Immunology. 128:e442-50.
- Lee GT, et al. 2011. Mol Immunol. 48:1540-7.
- Gene ID
- 654 View all products for this Gene ID
- UniProt
- View information about BMP-6 on UniProt.org
Other Formats
View All BMP-6 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Ultra-LEAF™ Purified anti-human BMP-6 | A20139D | Neut |
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Ultra-LEAF™ Purified anti-human BMP-6
Recombinant human BMP-6 (Cat. No. 595502) (black circles) in...