- Clone
- A17135E (See other available formats)
- Regulatory Status
- RUO
- Isotype
- Mouse IgG2b, κ
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Recombinant human BMP-7 (Cat. No. 595602) (black circles) induces the differentiation of ATDC5 mouse chondrogenic cells. Ultra-LEAF™ purified anti-human BMP-7 antibody (clone A17135E) (purple squares) inhibits the effect of recombinant human BMP-7 at 0.2 µg /mL on ATDC5 cells in a dose- dependent manner, whereas the GoInVivo™ purified mouse IgG2b, κ isotype ctrl antibody (gray triangles) (Cat. No. 400366) does not have the effect. ND50 range: 0.3 - 1.5 μg/mL.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 946903 | 100 µg | $358.00 | |||
| 946904 | 1 mg | $1205.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
Bone morphogenetic proteins (BMPs) belong to the transforming growth factor beta (TGF-β) superfamily. BMPs play a key role in embryonic development, especially during heart, neural and cartilage development. Around 20 BMP family members have been identified and characterized. BMPs signal through serine/threonine kinase receptors, and are composed of type I and II subtypes. Four type I receptors have been identified: type IA and IB BMP receptors, type IA activin receptor, and activin receptor-like kinase I. Three type II receptors have also been recognized: type II BMP receptor and type II and IIB activin receptors. BMP-7, originally identified by its properties to induce bone formation, has been identified as an anti-fibrotic molecule, antagonizing TGF-β1. Fibrosis is associated with the emergence of fibroblasts derived from endothelial cells, suggesting an endothelial-mesenchymal transition (EndMT). TGF-β1 induces endothelial cells to undergo EndMT, whereas bone BMP-7 preserved the endothelial phenotype. BMP-7 inhibits fibrosis in the kidney, lung, liver, heart, peritonium, oral submucosa tissue, and colonic wall.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human BMP-7
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
Block - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by neutralization of differentiation induced by recombinant human BMP-7 (Cat. No. 595602) at 0.2 µg/mL on ATDC5 mouse chondrogenic cells. ND50 range: 0.3-1.5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2894547 (BioLegend Cat. No. 946903)
AB_2894547 (BioLegend Cat. No. 946904)
Antigen Details
- Structure
- Growth factor, homodimer
- Distribution
-
Widely expressed
- Function
- BMP-7 promotes bone formation; BMP-7 induces Langerhans cell differentiation and proliferation, induces brown adipocyte differentiation and thermogenesis, and it has antifibrotic properties.
- Interaction
- Osteoblasts, Langerhans cells, adipocytes, endothelial cells
- Ligand/Receptor
- BMP type-I receptor ALK3 and Type II BMP receptor
- Cell Type
- Embryonic Stem Cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Neural Stem Cells
- Biology Area
- Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
- Molecular Family
- Cytokine/Chemokine Receptors, Growth Factors, Tumor Suppressors
- Antigen References
-
- Chen D, et al. 2004. Growth Factors 22:233.
- Tseng Y, et al. 2008. Nature 454:1000.
- Zeisberg E, et al. 2007. Nat. Med. 13:952.
- Hao ZM, et al. 2012. Mol. Ther. 20:2043.
- Yasmin N, et al. 2013. J. Exp. Med. 210:2597.
- Gene ID
- 655 View all products for this Gene ID
- UniProt
- View information about BMP-7 on UniProt.org
Other Formats
View All BMP-7 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Ultra-LEAF™ Purified anti-human BMP-7 Antibody | A17135E | Neut |
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Ultra-LEAF™ Purified anti-human BMP-7 Antibody
Recombinant human BMP-7 (Cat. No. 595602) (black circles) in...