- Clone
- A16056E (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Eotaxin, eotaxin-1
- Isotype
- Mouse IgG2a, κ
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Recombinant human CCL11 (Eotaxin) (Cat. No. 583002) (black circles) induces the chemotaxis of mouse Baf3-mCCR3 transfectant chemoattractant cells. Ultra-LEAF™ purified anti-human CCL11 (Eotaxin) (A16056E) (purple squares) neutralizes the chemotaxis of recombinant human CCL11 (Eotaxin) on mouse Baf3-mCCR3 cells in a dose-dependent manner, whereas the Ultra-LEAF™ purified mouse IgG2a, κ isotype control (Cat. No. 400264) (gray triangles) does not have the effect. ND50 range: 0.1 - 0.5 µg/mL.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 601753 | 100 µg | $358.00 | |||
| 601754 | 1 mg | $1205.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CCL11 is a member of the CC chemokine family and was originally identified as a chemoattractant for eosinophils. In response to allergens, CCL11, which participates in a variety of allergic diseases, promotes accumulation of eosinophils. In addition, CCL11 facilitates proliferation and metastasis of tumor cells expressing the CCL11 receptor, CCR3. CCL11 has been shown to induce angiogenic response by CCR3-positive microvascular endothelial cells.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human CCL11 (Eotaxin)
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
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Neut - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by neutralizing the chemotaxis induced by recombinant human CCL11 (Eotaxin) (Cat. No. 583002) on mouse Baf3-mCCR3 transfectant chemoattractant cells. ND50 range: 0.1 - 0.5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
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AB_2922630 (BioLegend Cat. No. 601753)
AB_2922630 (BioLegend Cat. No. 601754)
Antigen Details
- Structure
- Chemokine
- Distribution
-
Epithelial cells, smooth muscle cells, dendritic cells, and fibroblasts
- Function
- CCL11 selectively recruits eosinophils through binding to the chemokine receptor CCR3 during allergic response. CCL11 production is upregulated in response to several proimflammatory cytokines, such as IL-4, IL-13, TNF-α, and IFN-α, produced by mast cells and Th2 lymphocytes. CCL11 has been reported to stimulate eosinophil release from bone marrow, resulting in an elevated level of circulating eosinophils.
- Interaction
- Eosinophils
- Ligand/Receptor
- CCR3
- Cell Type
- Dendritic cells
- Biology Area
- Angiogenesis, Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
- Conroy DM, et al. 2001. Respir Res. 2:150-6.
- Levina V, et al. 2009. Clin Cancer Res. 15:2647-56.
- Salcedo R, et al. 2001. J Immunol. 166:7571-8.
- Kaehler J, et al. 2006. J Investig Med. 54:446-54.
- Hadjicharalambous C, et al. 2004. J Allergy Clin Immunol. 113:657-62.
- Crump MP, et al. 1998. J Biol Chem. 273:22471-9.
- Gene ID
- 6356 View all products for this Gene ID
- UniProt
- View information about CCL11 on UniProt.org
Other Formats
View All CCL11 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Ultra-LEAF™ Purified anti-human CCL11 (Eotaxin) | A16056E | Neut |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Ultra-LEAF™ Purified anti-human CCL11 (Eotaxin)
Recombinant human CCL11 (Eotaxin) (Cat. No. 583002) (black c...