- Clone
- A15150G (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Monocyte chemotactic protein 2 (MCP-2), C-C Motif Chemokine Ligand 8
- Isotype
- Mouse IgG2a, κ
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Recombinant human CCL8 (Cat. No. 581602) ( black circles) induces the chemotaxis of human BaF3-hCCR1 cells. Anti-human CCL8 antibody (clone W15150G) (purple squares) neutralizes the chemotaxis of recombinant human CCL8 on human BaF3-hCCR1 cells in a dose-dependent manner while the Ultra-LEAF™ purified mouse IgG2a, κ isotype ctrl antibody (Cat. No. 400264) (grey triangles) does not have the effect. ND50 range: 0.05 - 0.5 µg/mL.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 933203 | 100 µg | $270.00 | |||
| 933204 | 1 mg | $715.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CCL8, also known as MCP-2, is a beta chemokine initially isolated from human MG-63 osteosarcoma cells stimulated with IL-1β. CCL8 (MCP-2) has structural and functional similarity with MCP-1 and MCP-3, and it is often co-induced with those chemokines in mononuclear cells. IFN-γ is a strong inducer of CCL8 in monocytes and fibroblasts. CCL8 can be processed and secreted as a truncated chemokine CCL8 (6-75). This isoform does not chemoattract monocytes, but it binds to the CCR2 receptor and induces its internalization; as a result, CCL8 inhibits MCP-1 (CCL2) and MCP-2 (CCL8) ERK signaling and antagonizes the chemotactic activity of several CCR2 ligands (MCP-1/CCL2, MCP-2/CCL8, MCP-3/CCL7). CCL8 is highly expressed in the skin, and it serves as an agonist for the chemokine receptor CCR8, but not for CCR2. Therefore, it has been speculated that the CCL8-CCR8 interaction is a crucial regulator of Th2 cell homing, which drives IL-5-mediated chronic allergic inflammation. In addition, CCL8 binds CCR5 receptor with high affinity and inhibits the replication of human immunodeficiency virus type 1. CCL8 is highly expressed in different human diseases, and it is a potential biomarker for the diagnosis of graft-versus-host diseases and tuberculosis.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant Human CCL8
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
Neut - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by neutralization of the chemotaxis induced by recombinant human CCL8 on human BaF3-hCCR1 transfectants chemoattractant cells. ND50 range: 0.05 - 0.5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2861115 (BioLegend Cat. No. 933203)
AB_2861115 (BioLegend Cat. No. 933204)
Antigen Details
- Structure
- Chemokine
- Distribution
-
Fibroblast, endothelial cells, monocytes, and macrophages
- Function
- CCL8 chemoattracts monocytes, T cells, NK cells, mast cells, eosinophils, and basophils. CCL8 is induced by IL-1β, IFNβ, INFγ, dsRNA, virus, ConA, and anti-CD2. The induction of CCL8 by IFNγ synergizes with the TLR ligands peptidoglycan (TLR2), dsRNA (TLR3), and LPS (TLR4).
- Interaction
- Monocytes, T cells, NK cells, mast cells, eosinophils, and basophils
- Ligand/Receptor
- CCR1 (CD191), CCR2 (CD192), CCR3 (CD193), and CCR5 (CD195)
- Cell Type
- Basophils, Eosinophils, Mast cells, Monocytes, NK cells, T cells
- Biology Area
- Cell Biology, Neuroinflammation, Neuroscience, Signal Transduction
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
- Van Damme J, et al. 1992. J Exp Med. 176:59-65.
- Proost P, et al. 1998. J Immunol. 160:4034-41.
- Dean RA, et al. 2008. Blood. 112: 3455-64.
- Hori T, et al. 2008. Blood. 111:4403-12.
- Rom S, et al. 2010. FASEB J. 24:2292-300.
- Struyf S, et al. 2009. Eur J Immunol. 39:843-57.
- Islam SA, et al. 2011. Nat Immunol. 12:167-77.
- Gene ID
- 6355 View all products for this Gene ID
- UniProt
- View information about CCL8 on UniProt.org
Other Formats
View All CCL8 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Ultra-LEAF™ Purified anti-human CCL8 (MPC-2) | A15150G | Neut |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Ultra-LEAF™ Purified anti-human CCL8 (MPC-2)
Recombinant human CCL8 (Cat. No. 581602) ( black circles) in...