- Clone
- W20249H (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Colony Stimulating Factor 3 Receptor (CSF3R), Granulocyte Colony-Stimulating Factor Receptor (GCSFR), CD114, CD114 Antigen, G-CSF-R, SCN7
- Isotype
- Rat IgG2b, κ
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Recombinant human G-CSF Receptor/CD114 (Cat. No. 769902) inhibits M-NFS-60 cell proliferation induced by human G-CSF (Cat. No. 578604) (black circles) at 0.4 ng/mL. Ultra- LEAF™ purified anti-human CD114 (G-CSFR) (clone W20249H) (purple squares) neutralizes the inhibitory effect of recombinant human G-CSF Receptor/CD114 induced by human G-CSF in M-NFS-60 cells in a dose-dependent manner, whereas GoInVivo™ purified Rat IgG2b, κ isotype control (Cat. No. 400666) (gray triangles) does not have effect. ND50 range: 0.04 - 0.3 µg/mL.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 629653 | 100 µg | $360.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
Human G-CSF receptor (CSF3R) was initially isolated from the cDNA libraries of human U937 leukemia cells. The human G-CSF receptor is a type I cytokine receptor, possesses 836 amino acids, and has a clear homology (62.5%) with its murine counterpart. G-CSFR contains a conserved cytokine receptor homologous (CRH) domain, an Ig-like domain and three fibronectin type III-like domains in the extracellular region; a transmembrane region; and an intracellular region lacking intrinsic catalytic activity. Human G-CSF soluble receptor has been identified this form has a deletion of the transmembrane domain. The complex G-CSF/G-CSFR has a 2:2 ligand:receptor stoichiometry. G-CSFR stimulates signals transduction proteins that induce granulocytic proliferation and differentiation such as the STAT 1, 3 and 5 transcription factors, the Ras/Mek/Erk1/2 pathway, the Src-related kinases Lyn and Hck, the serine/threonine kinase Akt, and the Syk tyrosine kinas. Also, G-CSFR is associated to pathways that negatively regulate granulopoiesis, including those mediated by the SHP-1 tyrosine phosphatase and the suppressor of cytokine signaling 3 (SOCS3) protein. Different mutations in the gene encoding the G-CSFR have been described with clinical consequences related to the myeloid lineage, including severe congenital neutropenia (SCN), myelodysplastic syndrome (MDS), acute myeloid leukemia (AML), and chronic neutrophilic leukemia (CNL).
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant Human G-CSF Receptor/CD114
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
Neut - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by inhibition effect of recombinant human G-CSF Receptor/CD114 (Cat. No. 769902) at 0.1 µg/mL induced by human G-CSF (Cat. No. 578604) at 0.4 ng/mL in M-NFS-60 cells. ND50 range: 0.04 - 0.3 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_3097542 (BioLegend Cat. No. 629653)
Antigen Details
- Structure
- Cytokine receptor
- Distribution
-
Neutrophils, hematopoietic progenitor cells, placenta neurons, adult neural stem cells, endothelial cells, and cardiomyocytes
- Function
- Controls production, differentiation, and function of granulocytes. Integrin α9β1 augments receptor signaling and granulopoiesis in vivo.
- Interaction
- G-CSFR interacts with integrin α9β1.
- Ligand/Receptor
- G-CSF
- Cell Type
- Embryonic Stem Cells, Hematopoietic stem and progenitors
- Biology Area
- Stem Cells
- Molecular Family
- Cytokine/Chemokine Receptors, Soluble Receptors
- Antigen References
-
- Fukunaga R, et al. 1990. Proc Natl Acad Sci USA. 87:8702-6.
- Larsen A, et al. 1990. J Exp Med. 172:1559-70.
- Tamada T, et al. 2006. Proc Natl Acad Sci USA. 103:3135-40.
- Chen C, et al. 2006. Immunity. 25:895-906.
- Panopoulos AD & Watowich SS. 2008. Cytokine. 42:277-88.
- Liongue C & Ward AC. 2014. Front Oncol. 4:93.
- Gene ID
- 1441 View all products for this Gene ID
- UniProt
- View information about CD114 on UniProt.org
Other Formats
View All CD114 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Ultra-LEAF™ Purified anti-human CD114 (G-CSFR) | W20249H | Neut |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Ultra-LEAF™ Purified anti-human CD114 (G-CSFR)
Recombinant human G-CSF Receptor/CD114 (Cat. No. 769902) inh...