- Clone
- A3C6E2 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- HLDA6
- Other Names
- Stem cell factor receptor, c-kit, mast cell growth factor receptor, steel factor receptor
- Isotype
- Mouse IgG1, κ
-
Human erythroleukemic cell line TF-1 stained with Ultra-LEAF™ purified A3C6E2, followed by biotinylated anti-mouse IgG and Sav-PE
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 323417 | 100 µg | $270.00 | |||
| 323418 | 1 mg | $743.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CD117 is a 145 kD protein tyrosine kinase also known as c-kit. It is a receptor for stem cell factor or c-kit ligand. CD117 is expressed on pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, and acute myeloid leukemic cells (AML). CD117 binding of c-kit ligand induces phosphorylation of CD117, and stimulates proliferation and survival of primitive hematopoietic stem cells as well as erythroid-committed and granulo-monocytic committed cells. The A3C6E2 antibody potently blocks the binding of SCF.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- MOLM-1 megakaryocitic cell line
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
Block - Reported in the literature - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: Blocking the binding of SCF. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 323417 & 323418).
- RRID
-
AB_2888744 (BioLegend Cat. No. 323417)
AB_2888744 (BioLegend Cat. No. 323418)
Antigen Details
- Structure
- Growth factor receptor with tyrosine kinase activity, subclass III, approximately 145 kD
- Distribution
-
Pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, acute myeloid leukemic cells (AML)
- Function
- Growth factor receptor for stem cell factor. Induces proliferation and survival of primitive hematopoietic progenitors. Potent inducer of proliferation in erythroid-committed progenitor cells. Defects in CD117 have been linked to severe anemia and a decre
- Ligand/Receptor
- c-Kit ligand
- Modification
- Multiple phosphorylation sites
- Cell Type
- Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
1. Giebel LB, et al. 1992. Oncogene 7:2207.
2. Furitsu T, et al. 1993. J. Clin. Invest. 92:1736. - Gene ID
- 3815 View all products for this Gene ID
- UniProt
- View information about CD117 on UniProt.org
Other Formats
View All CD117 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human CD117 (c-kit) | A3C6E2 | FC |
| Biotin anti-human CD117 (c-kit) | A3C6E2 | FC |
| PE anti-human CD117 (c-kit) | A3C6E2 | FC |
| PE/Cyanine5 anti-human CD117 (c-kit) | A3C6E2 | FC |
| PE/Cyanine7 anti-human CD117 (c-kit) | A3C6E2 | FC |
| PerCP/Cyanine5.5 anti-human CD117 (c-kit) | A3C6E2 | FC |
| Ultra-LEAF™ Purified anti-human CD117 (c-kit) | A3C6E2 | FC,Block |
| TotalSeq™-D1260 anti-human CD117 (c-kit) | A3C6E2 | PG |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD117 (c-kit)
Human erythroleukemic cell line TF-1 stained with purified A... -
Biotin anti-human CD117 (c-kit)
Human erythroleukemia cell line TF-1 stained with biotinylat... -
PE anti-human CD117 (c-kit)
Human erythroleukemia cell line TF-1 stained with A3C6 PE -
PE/Cyanine5 anti-human CD117 (c-kit)
Human erythroleukemia cell line TF-1 stained with A3C6 PE/Cy... -
PE/Cyanine7 anti-human CD117 (c-kit)
Human erythroleukemia cell line TF-1 stained with A3C6 PE/Cy... -
PerCP/Cyanine5.5 anti-human CD117 (c-kit)
Human erythroleukemia cell line, HEL, was stained with CD117... -
Ultra-LEAF™ Purified anti-human CD117 (c-kit)
Human erythroleukemic cell line TF-1 stained with Ultra-LEAF... -
TotalSeq™-D1260 anti-human CD117 (c-kit)