- Clone
- W18195E (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Stem cell factor receptor, c-kit, mast cell growth factor receptor, steel factor receptor
- Isotype
- Rat IgG2a, λ
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Kasumi-3 cells (an acute myeloblastic leukemia cell line) were stained with Ultra-LEAF™ purified anti-human CD117 (c-kit) (clone W18195E) (filled histogram) or purified rat IgG2a, κ isotype control (open histogram), followed by PE goat anti-rat IgG. -
MO7E cells (a human megakaryocyte cell line) were pretreated with Ultra-LEAF™ purified anti-human CD117 (c-kit) (clone W18195E) (filled histogram) or purified rat IgG2a, κ isotype control (open histogram) for 15 minutes, then 100 ng of Recombinant Human SCF (Cat. No. 573902) were added for another 5 minutes in 37°C incubation. Cell were then fixed and permeabilized with Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803), and intracellularly stained with APC anti-ERK1/2 Phospho (Thr202/Tyr204) (Cat. No. 369521).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 380203 | 100 µg | $270.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CD117 is a 145 kD protein tyrosine kinase also known as c-Kit. It is a receptor for stem cell factor or c-Kit ligand. CD117 is expressed on pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, and acute myeloid leukemia cells (AML). CD117 binding of c-Kit ligand induces phosphorylation of CD117 and stimulates proliferation and survival of primitive hematopoietic stem cells as well as erythroid-committed and granulo-monocytic committed cells.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Human CD117 transfectant
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
Block - Verified
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Clone W18195E can complete block binding of clones H18022H and W18195D on target cells.
Clone W18195E does not affect binding of clones 104D2, W18195C or S18022G on target cells.
Clone W18195E can block ligand binding on c-kit. - RRID
-
AB_2924570 (BioLegend Cat. No. 380203)
Antigen Details
- Structure
- Growth factor receptor with tyrosine kinase activity, subclass III, approximately 145 kD
- Distribution
-
Pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, acute myeloid leukemic cells (AML)
- Function
- Receptor for stem cell factor. Induces proliferation and survival of primitive hematopoietic progenitors. Potent inducer of proliferation in erythroid-committed progenitor cells. Defects in CD117 have been linked to severe anemia and a decreased number of hematopoietic progenitor cells.
- Ligand/Receptor
- Stem cell factor (c-kit ligand)
- Cell Type
- Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
- Giebel LB, et al. 1992. Oncogene. 7:2207-17.
- Furitsu T, et al. 1993. J Clin Invest. 92:1736-44.
- Gene ID
- 3815 View all products for this Gene ID
- UniProt
- View information about CD117 on UniProt.org
Other Formats
View All CD117 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Ultra-LEAF™ Purified anti-human CD117 (c-kit) | W18195E | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Ultra-LEAF™ Purified anti-human CD117 (c-kit)
Kasumi-3 cells (an acute myeloblastic leukemia cell line) we... 
MO7E cells (a human megakaryocyte cell line) were pretreated...