- Clone
- W18063F (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CD137, CDw137, 4-1BB, 4-1BB ligand receptor, T cell antigen ILA, T cell antigen 4-1BB homolog
- Isotype
- Rat IgG2a, κ
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Recombinant human TNFRSF9/4-1BB-Fc Chimera (Cat. No. 772606) (black circles) binds to immobilized recombinant human TNFSF9 (Cat. No. 750006). Ultra-LEAF™ purified anti-human CD137 (4-1BB) antibody (clone W18063F) (purple squares) inhibits the binding in a dose-dependent manner, whereas the GoInVivo™ purified rat IgG2a, κ isotype ctrl antibody (Cat. No. 400562) (gray triangles) does not have the effect. This antibody blocks the binding of 0.03 µg/mL recombinant human TNFRSF9/4-1BB-Fc Chimera to 0.5 µg/mL of immobilized recombinant human TNFSF9 with an ND50 = 0.01 - 0.06 µg/mL.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 944903 | 100 µg | $358.00 | |||
| 944904 | 1 mg | $1205.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
TNFRSF9, also known as 4-1BB and CD137, is a member of the tumor necrosis factor receptor superfamily (TNFRSF). It is a costimulatory receptor similar to OX40, CD27, CD30, and CD40. The interaction of TNFRSF9 and its ligand, TNFSF9, leads to the activation of cytotoxic T cells, reactivation of anergic T lymphocytes, and induces T cell proliferation. TNFRSF9/TNFSF9 preferentially enhances the expansion of CD8+ T cells rather than CD4+ T cells, through the increase of IL-2Rα (CD25) and IL-2 expressions in CD8+ T cells. Among the TNFRSF members, TNFRSF9 is the most potent inducer of IL-2Rα expression on CD8+ T cells. In vivo studies with TNFSF9 knockout mice and with the agonistic anti-TNFRSF9 monoclonal antibody showed that TNFRSF9/TNFSF9 interaction keeps the optimal CD8+ T cell-mediated immune response against viral infections and tumors. The mouse TNFRSF9 has 60% identity to the human receptor at the amino acid level.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant human CD137
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
Block - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by blocking the binding between human TNFSF9 (Cat. No. 750006) and human TNFRSF9/4-1BB (Cat. No. 772606). The ND50 range: 0.01 - 0.06 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
-
- RRID
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AB_2904454 (BioLegend Cat. No. 944903)
AB_2904454 (BioLegend Cat. No. 944904)
Antigen Details
- Structure
- Dimer
- Distribution
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Activated T cells, natural killer cells, macrophages, neutrophils, dendritic cells, eosinophils, Tregs, activated B cells, mast cells, and endothelial cells in tumor capillaries
- Function
- Proliferation of T cells, activates their effector functions, survival, and establishes immunological memory
- Interaction
- Activated antigen presenting cells, macrophages, B cells, B cell lymphoma, dendritic cells, a small fraction of T cells, epithelial, and endothelial cells.
- Ligand/Receptor
- TNFSF9 and CD137L
- Cell Type
- B cells, Dendritic cells, Endothelial cells, Mast cells, T cells, Tregs
- Biology Area
- Immuno-Oncology, Immunology, Protein Trafficking and Clearance
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors, Soluble Receptors
- Antigen References
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- Pollok KE, et al. 1994. Eur. J. Immunol. 24:367.
- Watts TH. 2005. Annu. Rev. Immunol. 23:23-68.
- Zhu Y, et al. 2007. Blood 109:4882.
- Locatelli F. 2009. Blood 11:2208.
- Wang J, et al. 2010. J. Immunol. 185:7654.
- Eun SY, et al. 2015. J. Immunol. 1:134-41.
- Spencer AJ, et al. 2014. PLoS One. 9:e105520.
- Yi L, et al. 2014. PLoS One 1:e86337.
- Gene ID
- 3604 View all products for this Gene ID
- UniProt
- View information about CD137 on UniProt.org
Other Formats
View All CD137 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Ultra-LEAF™ Purified anti-human CD137 (4-1BB) | W18063F | Block |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Ultra-LEAF™ Purified anti-human CD137 (4-1BB)
Recombinant human TNFRSF9/4-1BB-Fc Chimera (Cat. No. 772606)...