- Clone
- W22023G (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CD137L, 4-1BB-L, 4-1BBL, 41BBL, 4-1BB Ligand, 41BB Ligand
- Isotype
- Rat IgG2b, κ
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Biotinylated recombinant human TNFRSF9 (4-1BB)-Fc chimera (Cat. No. 772614) (black circles) binds to immobilized recombinant human TNFSF9 (4-1BBL) (Cat. No. 750006). Ultra-LEAF™ purified anti-human CD137L (4-1BB Ligand) (clone W22023G) (purple squares) inhibits the binding in a dose-dependent manner, whereas the Ultra-LEAF™ purified rat IgG2b, κ isotype control (Cat. No. 400544) (gray triangles) does not have effect. This antibody blocks the binding of 0.025 µg/mL biotinylated recombinant human TNFRSF9 (4-1BB)-Fc chimera to 1 µg/mL immobilized recombinant human TNFSF9 (4-1BBL). ND50 range: 0.01 - 0.07 µg/mL.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 647153 | 100 µg | $455.00 | |||
| 647154 | 1 mg | $1155.00 | |||
TNFSF9, also known as 4-1BBL and CD137L, is a type II transmembrane glycoprotein that belongs to the TNF superfamily. This protein is mainly expressed on antigen presenting cells (APC), and on B lymphocytes particularly. The interaction between TNFSF9 and its receptor, CD137, which is mainly expressed on T cells, leads to the activation of cytotoxic T cells. In addition, TNFSF9 (CD137) interaction can reactivate anergic T lymphocytes and induce T cell proliferation. This molecule plays a role in optimal CD8 T cell response. Interestingly, it appears that TNFSF9 is able to act as a costimulatory molecule without the engagement of other costimulatory molecules such as CD28. TNFSF9 signaling on T cells has also been shown to limit T cell activation. CD137L is upregulated in peripheral CD14+ monocytes of chronic hepatitis B patients and is associated with liver cirrhosis. Human TNFSF9 shares 36% of its amino acid sequence with its murine counterpart. Mice deficient of TNFSF9 are susceptible to develop B-cell lymphomas.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant Human TNFSF9 (4-1BBL)
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
Block - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by blocking the binding of 0.025 µg/mL biotinylated recombinant human TNFRSF9 (4-1BB)-Fc chimera (Cat. No. 772614) to immobilized 1 µg/mL recombinant human TNFSF9 (4-1BBL) (Cat. No. 750006). ND50 range: 0.01 - 0.07 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_3662329 (BioLegend Cat. No. 647153)
AB_3662329 (BioLegend Cat. No. 647154)
Antigen Details
- Structure
- Monomer
- Distribution
-
Constitutively expressed on a fraction of dendritic cells (DCs). Inducible on activated monocytes, macrophages, B cells, a small fraction of T cells, epithelial, and endothelial cells
- Function
- TNFSF9 plays roles in cytotoxic T cell activation, T cell proliferation, and apoptosis
- Interaction
- Activated T cells, natural killer cells (NK), macrophages, and dendritic cells
- Ligand/Receptor
- CD137
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors, Soluble Receptors
- Antigen References
-
- Vinay DS and Kwon BS. 1998. Semin. Immunol. 10:481-9.
- Watts TH. 2005. Annu Rev Immunol. 23:23:23-68.
- Pollok KE, et al. 1994. Eur J Immunol. 24:367-74.
- Wang J, et al. 2010. J Immunol. 185:367-74.
- Eun SY, et al. 2014. J Immunol. 194:134.
- Spencer AJ, et al. 2014. PLoS One 9:e105520.
- Locatelli F. 2009. Blood. 114:2208-9.
- Gene ID
- 8744 View all products for this Gene ID
- UniProt
- View information about CD137L on UniProt.org
Other Formats
View All CD137L Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Ultra-LEAF™ Purified anti-human CD137L (4-1BB Ligand) | W22023G | Block |
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Ultra-LEAF™ Purified anti-human CD137L (4-1BB Ligand)
Biotinylated recombinant human TNFRSF9 (4-1BB)-Fc chimera (C...