- Clone
- W21097B (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CD226, Platelet and T-cell activation antigen 1, PTA1, T lineage specific activation antigen 1 antigen
- Isotype
- Rat IgG2b, κ
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Biotinylated human CD155-Fc chimera (Cat. No. 796604) (black circles) binds to immobilized recombinant human DNAM-1/CD226-Fc chimera (Cat. No. 773606). Ultra-LEAF™ purified anti-human CD226 (DNAM-1) (clone W21097B) (purple squares) inhibits the binding in a dose-dependent manner, whereas the Ultra-LEAF™ purified rat IgG2b, κ isotype control (Cat. No. 400644) (gray triangles) does not have the effect. This antibody blocks the binding of 0.2 μg/mL biotinylated human CD155-Fc chimera to 1 µg/mL immobilized recombinant human DNAM-1/CD226-Fc chimera. ND50 range: 0.02 - 0.15 μg/mL.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 639753 | 100 µg | $440.00 | |||
| 639754 | 1 mg | $1145.00 | |||
DNAM-1, also known as CD226, was first identified as T lineage-specific activation antigen (TLiSA) and subsequently renamed as Platelet and T-cell activation antigen 1 (PTA). DNAM-1 consists of two Ig-like domains on its extracellular portion, a transmembrane region and a cytoplasmic region containing binding motifs for members of band 4.1 family and membrane-associated guanylate kinase homolog (MAGUK) family. DNAM-1 is predominantly expressed on cell surface of T cells, NK cells, monocytes/ macrophages, platelets and megakaryocytes and a subset of B cells. DNAM-1 serves as a signaling-transducing adhesion molecule, and is physically and functionally associates with LFA-1 (CD11a/CD18) on NK and T cells. The clustering of LFA-1 induces Fyn-mediated phosphorylation of DNAM-1 and signaling. Evidence suggests that DNAM-1 is involved in NK and T cell-mediated cytotoxicity (CTL) against ligand-expressing tumor cells. Two ligands for DNAM-1 have been identified, the poliovirus receptor (PVR/CD155) and its family member nectin-2 (PRR/CD112), which are located at cell junctions and broadly expressed on epithelial, endothelial and neuronal cells. Additionally, DNAM-1 regulates monocyte extravasation through PVR expressed by endothelial cells. It has been reported that DNAM-1 is specifically expressed on differentiated Th1 cells, but not to Th2 or Th0 cells. Antibody blocking assay demonstrates that DNAM-1 may play a role in polarization of T cells, activation and effectors of Th1 cells. PVRIG (CD112R) is identified as a novel immune checkpoint to inhibit T cell response by competing DNAM-1 with CD112.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant Human DNAM-1/CD226-Fc Chimera
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
Block - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by blocking the binding of 0.2 µg/mL recombinant biotinylated human CD155-Fc chimera (Cat. No 796604) to immobilized 1 µg/mL recombinant human DNAM-1/CD226-Fc chimera (Cat. No. 773606). ND50 range: 0.02 - 0.15 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_3662324 (BioLegend Cat. No. 639753)
AB_3662324 (BioLegend Cat. No. 639754)
Antigen Details
- Structure
- Disulfide bond-linked homodimer, Ig domain containing adhesion molecule
- Distribution
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Peripheral blood T cells, NK cells, monocytes/macrophages, platelets and megakaryocytes and a subset of B cells; plasma membrane
- Function
- It is involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cells.
- Interaction
- Epithelial, endothelial and neuronal cells; LFA-1
- Ligand/Receptor
- PVR (CD155) and Nectin-2 (CD112)
- Cell Type
- B cells, Macrophages, Monocytes, NK cells, T cells, Th1
- Biology Area
- Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
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- Fuchs A, Colonna M. 2006. Semin Cancer Biol. 16:359-66.
- Kojima H, et al. 2003. J Biol Chem. 278:36748-53.
- Burns GF, et al. 1985. J Exp Med. 161:1063-78.
- Scott JL, et al. 1989. J Biol Chem. 264:13475-82.
- Shibuya A, et al. 1996. Immunity. 4:573-81.
- Shibuya K et al. 1999. Immunity. 11:615-23.
- Ralston KJ, et al. 2004. J Biol Chem. 279:33816-28.
- Tahara-Hanaoka S, et al. 2006. Blood. 107:1491-6.
- Bottino C, et al. 2003. J Exp Med. 198:557.
- Reymond N, et al. 2004. J Exp Med. 199:1331-41.
- Dardalhon V, et al. 2005. J Immunol. 175:1558-65.
- Shibuya K, et al. 2003. J Exp Med. 198:1829-39.
- Gene ID
- 10666 View all products for this Gene ID
- UniProt
- View information about CD226 on UniProt.org
Other Formats
View All CD226 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Ultra-LEAF™ Purified anti-human CD226 (DNAM-1) | W21097B | Block |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Ultra-LEAF™ Purified anti-human CD226 (DNAM-1)
Biotinylated human CD155-Fc chimera (Cat. No. 796604) (black...