Ultra-LEAF™ Purified anti-human CD66c Antibody

Pricing & Availability
Clone
W20030E (See other available formats)
Regulatory Status
RUO
Other Names
Carcinoembryonic antigen-related cell adhesion molecule 6
Isotype
Rat IgG2a, κ
W20030E_ULEAF_CD66c_Antibody_1_013023
The human lung adenocarcinoma cell line A549 was grown on 96-well plates with coverslip bottom and were fixed with Fixation Buffer (Cat. No. 420801) for 30 minutes. Cells were washed twice with PBS and stained with 5 µg/mL Ultra-LEAF™ purified anti-human CD66c (clone W20030E) followed by Alexa Fluor® 555 anti-rat IgG (red) (Cat. No. 405420). Nuclei were counterstained with DAPI (blue) (Cat. No. 422801). Cells were imaged using a 40X objective.
  • W20030E_ULEAF_CD66c_Antibody_1_013023
    The human lung adenocarcinoma cell line A549 was grown on 96-well plates with coverslip bottom and were fixed with Fixation Buffer (Cat. No. 420801) for 30 minutes. Cells were washed twice with PBS and stained with 5 µg/mL Ultra-LEAF™ purified anti-human CD66c (clone W20030E) followed by Alexa Fluor® 555 anti-rat IgG (red) (Cat. No. 405420). Nuclei were counterstained with DAPI (blue) (Cat. No. 422801). Cells were imaged using a 40X objective.
  • W20030E_ULEAF_CD66c_Antibody_2_013023
    Human peripheral blood granulocytes were stained with Ultra-LEAF™ purified anti-human CD66c (clone W20030E) (filled histogram) or rat IgG2a, κ isotype control (open histogram) followed by anti-rat IgG PE.
  • W20030E_ULEAF_CD66c_Antibody_3_013023
    The effect of clone W20030E to induce CD66c signaling by cross-linking was tested on the human lung adenocarcinoma cell line A549. Cells were grown on 96-well plates with coverslip bottom and treated with 5 µg/mL Ultra-LEAF™ anti-human CD66c (clone W20030E) (left panels) or rat IgG2a, κ isotype control (right panels) for 15 minutes at 37°C followed by anti-rat IgG secondary antibody (5 µg/mL) for 30 minutes at 37°C. Cells were fixed with Fixation Buffer for 30 minutes. Cells were washed twice with 1X Intracellular Staining Permeabilization Wash Buffer and then stained with purified anti-AKT Phospho (Ser473) (clone A21001C) followed by Alexa Fluor® 555 anti-mouse IgG (yellow), Flash Phalloidin™ Red 594 (magenta) and DAPI (blue).
  • W20030E_ULEAF_CD66c_Antibody_4_013023
    The effect of Ultra-LEAF™ anti-human CD66c (clone W20030E) on the ability of cells to invade through the extracellular matrix was tested by a Matrigel invasion assay. The human lung adenocarcinoma cell line A549 was serum starved for 24h and seeded onto the top well of Corning® BioCoat Matrigel® Invasion Chamber. Rat IgG2a, κ isotype control antibody was used as a control. DMEM media with 20% serum was placed in the bottom well as chemoattractant. After 16h, cells that had migrated to the bottom of the membrane were counted.
  • W20030E_ULEAF_CD66c_Antibody_5_updated_021623
    The effect of Ultra-LEAF™ anti-human CD66c (clone W20030E) on the ability of cells to migrate was tested by a wound healing assay. The human lung adenocarcinoma cell line A549 was grown to confluence, and a line was scratched using a 10 µL pipette tip. Rat IgG2a, κ isotype control antibody was used as a control. After 16h, the width of the gap was measured using ImageJ, and % gap closure was calculated normalized to isotype controls.
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386103 100 µg $360.00
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386104 1 mg $1205.00
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Description

Carcinoembryonic antigen (CEA) Cell Adhesion Molecule 6 (CEACAM6) belongs to the (CEA) family whose members are glycosyl phosphatidyl inositol (GPI) anchored cell surface glycoproteins. As a cell adhesion molecule, CEACAM6 mediates homotypic binding with other CEA family members and heterotypic binding with integrin receptors. CEACAM6 is expressed on epithelial surfaces and in the myeloid lineage. Despite lacking transmembrane and intracellular domains, GPI-anchored proteins, including CEACAM family members, are able to influence transmembrane signaling via tyrosine kinases. CEACAM6 modulates c-Src tyrosine kinase activity. Overexpressed in more than 50% of all human adenocarcinomas, CEACAM6 promotes aberrant cell differentiation, anti-apoptosis, cell growth and resistance to therapeutic agents, cell invasion and metastasis.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant protein, aa 35-320
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

ICC - Quality tested

FC, Blocking, Activation - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 5.0 - 10.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2941566 (BioLegend Cat. No. 386103)
AB_2941566 (BioLegend Cat. No. 386104)

Antigen Details

Structure
Glycosyl phosphatidyl inositol (GPI) anchored cell surface glycoproteins Immunoglobulin superfamily
Distribution

Apical Cell Membrane

Function
The extracellular N-terminus Ig-like V-type domain is necessary for homophilic and heterophilic intercellular adhesion.
Interaction
Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM5 and CEACAM8
Cell Type
Epithelial cells, Granulocytes
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Duxbury MS, et al. 2004. Oncogene. 23:465-73.
  2. Oikawa S, et al. 1991. J Biol Chem. 266:7995-8001.
  3. Noworolska A, et al. 1986. Cancer Detect Prev. 9:365-71.
  4. Wu S, et al. 2021. Transl Oncol. 14:101057
Gene ID
4680 View all products for this Gene ID
UniProt
View information about CD66c on UniProt.org
Go To Top Version: 1    Revision Date: 01/27/2023

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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