- Clone
- QA18A16 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- B7-1, B7, BB1
- Isotype
- Mouse IgG1, κ
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Human B-cell Burkitt's lymphoma cell line Raji was stained with Ultra-LEAF™ purified CD80 recombinant (clone QA18A16) (filled histogram) or Ultra-LEAF™ purified mouse IgG1, κ isotype control (open histogram), followed by PE anti-mouse IgG.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 370613 | 100 µg | $220.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CD80, also known as B7-1, B7, and BB1, is a 60 kD single chain type I glycoprotein belonging to the immunoglobulin superfamily. CD80 is expressed on activated B and T cells, macrophages, and dendritic cells. CD80 binds to CD28 and CD152 (CTLA-4). Along with CD86, CD80 plays a critical role in regulation of T cell activation. The interaction of CD80 with CD28 provides a potent costimulatory signal for T cell activation through the CD3 complex, while its interaction with CTLA-4 provides an inhibitory signal for T cell activation.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2924562 (BioLegend Cat. No. 370613)
Antigen Details
- Structure
- Ig superfamily, single chain type I transmembrane glycoprotein, 60 kD
- Function
- T cell costimulation
- Ligand/Receptor
- CD28, CD152 (CTLA-4)
- Cell Type
- B cells, Dendritic cells, Macrophages, T cells, Tregs
- Biology Area
- Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
-
1. Kim GJ, et al. 2012. Immunol. 188:4217-4225.
- Gene ID
- 941 View all products for this Gene ID
- UniProt
- View information about CD80 on UniProt.org
Other Formats
View All CD80 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| PE anti-human CD80 Recombinant Antibody | QA18A16 | FC |
| Purified anti-human CD80 Recombinant Antibody | QA18A16 | FC |
| Ultra-LEAF™ Purified anti-human CD80 Recombinant Antibody | QA18A16 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-human CD80 Recombinant Antibody
Human B-cell Burkitt's lymphoma cell line Raji was stained w... -
Purified anti-human CD80 Recombinant Antibody
Human B-cell Burkitt's lymphoma cell line Raji was stained w... -
Ultra-LEAF™ Purified anti-human CD80 Recombinant Antibody
Human B-cell Burkitt's lymphoma cell line Raji was stained w...