- Clone
- W21100D (See other available formats)
- Regulatory Status
- RUO
- Other Names
- HUAT, Lectin, galactoside-binding, soluble, 9 (LGALS9), LGALS9A
- Isotype
- Rat IgG2b, κ
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Recombinant Human TIM-3-Fc chimera (Cat. No 759904) (black circles) binds to immobilized recombinant Human Galectin-9 (E. coli expressed) (Cat. No. 754806). Ultra-LEAF™ purified anti-human Galectin-9 (clone W21100D) (purple squares) inhibits the binding in a dose-dependent manner, whereas the Ultra-LEAF™ purified rat IgG2b, κ isotype control (Cat. No. 400644) (gray triangles) does not have effect. This antibody blocks the binding of 2 µg/mL human TIM-3-Fc chimera to 5 µg/mL immobilized recombinant human Galectin-9 (E. coli expressed). ND50 range: 0.5 - 3 µg/mL
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 637053 | 100 µg | $455.00 | |||
| 637054 | 1 mg | $1180.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
Galectins constitute a family of conserved animal lectins, which are characterized by their affinity for poly-N-acetyllactosamine-enriched glycoconjugates and sequence similarities in the carbohydrate recognition domain (CRD). In vertebrates, the Galectin family comprises 15 members. Galectins have been classified into three types: a) Prototype monomeric Galectins with a single polypeptide chain that dimerize (Galectin-1, -2, -5, -7, -10, -11, -13 -14, and -15); b) Tandem repeat galectins composed of a single polypeptide chain with two CRDs, joined by a linker peptide (Galectin-4, -6, -8, -9, and -12); and c) Chimeric galectin, thus far containing only Galectin-3, which consists of one C-terminal CRD linked to an N-terminal domain. Galectin-9 may be retained intracellularly or transported to the cell surface where it can be cleaved to generate a soluble form. Galectin-9 functions in effector and regulatory phases of the immune response. During inflammation, the production of Galectin-9 in vivo generates a microenvironment that limits effector T cell responses. Galectin-9 can negatively regulate the Th1 immune response by engaging TIM-3 on T cells, which leads to induction of intracellular Ca2+ influx and activation of apoptosis pathways. In addition, Galectin-9 has been shown to function as a urate transporter in the kidney. Galectin-9 has been identified as a possible prognostic marker in breast cancer, melanoma, and oral squamous cell carcinomas.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant human Galectin-9
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
Block - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by blocking the binding of 2 µg/mL recombinant human TIM-3-Fc chimera (Cat. No 759904) to immobilized 5 µg/mL recombinant human Galectin-9 (E. coli expressed) (Cat. No. 754806). ND50 = 0.5 – 3 μg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_3106077 (BioLegend Cat. No. 637053)
AB_3106077 (BioLegend Cat. No. 637054)
Antigen Details
- Structure
- Homodimer
- Distribution
-
Galectin-9 is expressed by lymphocytes, dendritic cells, neutrophils, eosinophils, astrocytes, endothelial cells, fibroblasts, thymus stromal cells, and epithelial cells.
- Function
- Cell aggregation, adhesion, chemotaxis, apoptosis, suppression of Th1 and Th17 responses, and induction of regulatory T cells. IFN-γ induces galectin-9 expression in fibroblasts, endothelial, and Kupffer cells. IL-1β and IL-5 induces it in astrocytes and eosinophils, respectively.
- Interaction
- Extracellular matrix
- Ligand/Receptor
- Galectin-9 binds to glycans featuring galactose and its derivatives: CD44 and TIM-3.
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Stem Cells
- Molecular Family
- Adhesion Molecules, Immune Checkpoint Receptors
- Antigen References
-
- Toscano MA, et al. 2009. Nat Immunol Rev. 9:338-52.
- Hadari YR, et al. 2000. J Cell Sci. 113:2385-52.
- Tsuboi Y, et al. 2007. Clin Immunol. 124:221-33.
- Zhu C, et al. 2005. Nat Immunol. 6:1245-52.
- Wu C, et al. 2014. Immunity. 41:270-82.
- Graessler J, et al. 2000. Adv Exp Med Biol. 486:179-83.
- Bacigalupo ML, et al. 2013. World J Gastroenterol. 47:8831-49.
- Gene ID
- 3965 View all products for this Gene ID
- UniProt
- View information about Galectin-9 on UniProt.org
Other Formats
View All Galectin-9 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Ultra-LEAF™ Purified anti-human Galectin-9 | W21100D | Block |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Ultra-LEAF™ Purified anti-human Galectin-9
Recombinant Human TIM-3-Fc chimera (Cat. No 759904) (black c...