Ultra-LEAF™ Purified anti-human IFN-γ Antibody

Pricing & Availability
Clone
B27 (See other available formats)
Regulatory Status
RUO
Other Names
Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-g, IFN-gamma
Isotype
Mouse IgG1, κ
B27_Purified_IFN-gamma_Antibody_012519_updated.png
PMA+ionomycin-stimulated human peripheral blood lymphocytes (6-hours), in the presence of monensin, were fixed, permeabilized, and then intracellularly stained with IFN-γ (clone B27) Purified (filled histogram), or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram) followed by anti-mouse IgG FITC.
  • B27_Purified_IFN-gamma_Antibody_012519_updated.png
    PMA+ionomycin-stimulated human peripheral blood lymphocytes (6-hours), in the presence of monensin, were fixed, permeabilized, and then intracellularly stained with IFN-γ (clone B27) Purified (filled histogram), or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram) followed by anti-mouse IgG FITC.
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Cat # Size Price Quantity Check Availability
506531 100 µg $110.00
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506532 1 mg $149.00
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506533 5 mg $539.00
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506534 25 mg $1408.00
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506535 50 mg $2354.00
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506536 100 mg $3383.00
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Description

Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells. The B27 antibody reacts with the human interferon-γ. The B27 antibody can neutralize the bioactivity of natural or recombinant IFN-γ.

Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee, Baboon, Cynomolgus, Rhesus, African Green, Pigtailed Macaque, Sooty Mangabey
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
E. coli-expressed recombinant human IFN-γ
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

ICFC - Quality tested
FC, Neut - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Flow Cytometry2: The fluorochrome-labeled B27 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-? -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.
Neutralization1,3,6,7: The Ultra-LEAF™ Purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IFN-? bioactivity (Cat. No. 506531).

Application References

(PubMed link indicates BioLegend citation)
  1. Favre C, et al. 1989. Molec. Immunol. 26:17. (Neut)
  2. Kaur A, et al. 2002. J Virol. 76:3646.
  3. Abrams J, et al. 1992. Immunol. Rev. 127:5. (Neut)
  4. Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
  5. Rout N, et al. 2010. PLoS One 5:e9787. (FC)
  6. Acosta-Rodriguez EV, et al. 2007. Nat. Immunol. 9:942-9. (Neut)
  7. Gangur V, et al. 1998. FASEB J. 12:705-13. (Neut)
Product Citations
  1. Asashima H, et al. 2023. Cell Rep. 42:111895. PubMed
  2. Gruber CN, et al. 2020. Cell. 183:982. PubMed
  3. Bhojnagarwala PS, et al. 2022. Mol Ther Oncolytics. 26:289. PubMed
  4. OConnor RA, et al. 2021. OncoImmunology. 10(1):1940675. PubMed
  5. Jiang J, et al. 2021. Front Immunol. 12:785549. PubMed
RRID
AB_2801091 (BioLegend Cat. No. 506531)
AB_2801091 (BioLegend Cat. No. 506532)
AB_2801091 (BioLegend Cat. No. 506533)
AB_2801091 (BioLegend Cat. No. 506534)
AB_2801091 (BioLegend Cat. No. 506535)
AB_2801091 (BioLegend Cat. No. 506536)

Antigen Details

Structure
Cytokine; dimer; 20-25 kD (Mammalian)
Bioactivity
Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APC
Cell Sources
CD8+ and CD4+ T cells, NK cells
Cell Targets
T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Receptors
IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571.
4. Gray P, et al. 1987. Lymphokines 13:151.

Regulation
Upregulated by IL-2, FGF-basic, EGF; downregulated by vitamin D3 or DMN; labile at pH2
Gene ID
3458 View all products for this Gene ID
UniProt
View information about IFN-gamma on UniProt.org
Go To Top Version: 5    Revision Date: 12/16/2021

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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