- Clone
- W19171A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF)
- Isotype
- Rat IgG2b, κ
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Recombinant human IFN-γ (Cat. No. 570206) (black circles) decreased the cell viability of HT-29 cells with recombinant human TNF-α (Cat. No. 570106) at 0.1 µg/mL. Ultra-LEAF™ purified anti-human IFN-γ (clone W19171A) (purple squares) reverses the effect of recombinant human IFN-γ at 0.08 µg/mL and recombinant human TNF-α at 0.1 µg/mL on HT-29 cells in a dose-dependent manner, whereas the Ultra-LEAF™ purified rat IgG2b, κ isotype control (Cat. No. 400644) (gray triangles) does not have the effect. ND50 range : 1 - 5 μg/mL
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 611853 | 100 µg | $358.00 | |||
| 611854 | 1 mg | $1205.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ demonstrates anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant human IFN-γ
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
Neut - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by inhibiting the cytotoxity induced by recombinant human IFN-γ (Cat. No. 570206) at 0.08 μg/mL and recombinant human TNF-α (Cat. No. 570106) at 0.1 μg/mL on HT-29 cells. ND50 range: 1.0 - 5.0 μg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2941603 (BioLegend Cat. No. 611853)
AB_2941603 (BioLegend Cat. No. 611854)
Antigen Details
- Structure
- Cytokine, dimer
- Distribution
-
CD8+ and CD4+ T cells, NK cells
- Function
- Upregulated by IL-2, FGF-basic, EGF; downregulated by vitamin D3 or DMN; labile at pH2
- Interaction
- T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
- Ligand/Receptor
- IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
- Cell Type
- NK cells, T cells
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Stem Cells
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
- Fitzgerald K, et al. 2001. The Cytokine FactsBook Academic Press San Diego.
- De Maeyer E, et al. 1992. Curr Opin Immunol. 4:321-6.
- Farrar M, et al. 1993. Annu Rev Immunol. 11:571-611.
- Gray P, et al. 1987. Lymphokines. 13:151.
- Gene ID
- 3458 View all products for this Gene ID
- UniProt
- View information about IFN-gamma on UniProt.org
Other Formats
View All IFN-γ Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Ultra-LEAF™ Purified anti-human IFN-γ | W19171A | Neut |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Ultra-LEAF™ Purified anti-human IFN-γ
Recombinant human IFN-γ (Cat. No. 570206) (black circles) de...