- Clone
- A16057A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- TIFa, IL-D110, IL-TIF, TIFIL-23, zcyto18
- Isotype
- Mouse IgG2a, κ
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Recombinant human IL-22 (Black circles, Cat. No. 571306) stimulates the production of IL-10 in human Colo 205 cells. Anti-human IL-22 antibody (clone A16057A, purple squares) inhibits the effect of recombinant human IL-22 at 0.75 ng/mL in human Colo 205 cells in a dose-dependent manner. The ND50 = 1.2 - 6 μg/mL.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 618303 | 100 µg | $270.00 | |||
| 618304 | 1 mg | $715.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
IL-22 is a cytokine structurally related to IL-10. Originally identified as a murine gene induced by IL-9 in T and mast cells, IL-22 was initially designated ILTIF (IL-10-related T cell-derived inducible factor). IL-22 belongs to a family of cytokines with limited homology to IL-10, namely IL-10, IL-19, IL-20, IL-24, IL-26, and the IFN-λs, IL-28A, IL-28B and IL-29. Human IL-22 shares 79% amino acid identity with murine IL-22 and 25% identity with human IL-10. IL-22 biological activity is initiated by binding to a cell surface complex composed of IL-22R1 and IL-10R2 receptor chains. Activity is further regulated through interactions with the soluble binding protein, IL-22BP, which shares sequence similarity with an extracellular region of IL-22R1 (sIL-22R1). Both chains of the IL-22R complex belong to the class II CRF. Two types of IL-22 binding receptors have been discovered, a membrane-bound receptor and a soluble receptor, both encoded by different genes. IL-22 is produced by Th17 cells and newly identified Th22 cells. It plays a critical role in mucosal immunity in addition to the deregulated inflammation observed in autoimmune diseases.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant Human IL-22
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
Neut - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by the neutralization of IL-10 production induced by human recombinant IL-22 (0.75 ng/mL) on human Colo 205 cells. ND50 range: 1.2 - 6.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2814466 (BioLegend Cat. No. 618303)
AB_2814466 (BioLegend Cat. No. 618304)
Antigen Details
- Structure
- Cytokine
- Distribution
-
Thymic lymphomas, T cells, activated T cells, macrophages, and mast cells
- Function
- Enhances the production of acute phase proteins in a hepatocyte cell line, HepG2; Promotes Th17 cells to transmigrate through the blood-brain barrier in multiple sclerosis
- Interaction
- Interacts with a cell surface complex composed of IL-22R1 and IL-10R2 receptor chains which are expressed in epithelial cells of inflammatory diseases such as psoriasis, Crohn's disease, rheumatoid arthritis, or allergic asthma
- Ligand/Receptor
- IL-22R1 and IL-10R2 receptor chains
- Cell Type
- Epithelial cells, NK cells, T cells, Th17
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Stem Cells
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
- Nagalakshmi ML, et al. 2004. Intern Immunopharmacol. 4:679-691.
- Kebir H, et al. 2007. Nat Med. 13:1173-1175.
- Gu Y, et al. 2008. Eur J Immunol. 38:1807-1813.
- Pene J, et al. 2008. J Immunol. 180:7423-7430.
- Dumotier L, et al. 2000. J immune 164:1814-1819.
- Xie MH, et al. 2000. J Biol Chem. 765:31335-31339.
- Jones BC, et al. 2008. Structure. 16:1333-44
- Kotenko SV, et al. 2001. J Immunol. 166:7096-71033.
- Chang C, et al. 2006. Cell Res 16:902-907.
- Gene ID
- 50616 View all products for this Gene ID
- UniProt
- View information about IL-22 on UniProt.org
Other Formats
View All IL-22 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Ultra-LEAF™ Purified anti-human IL-22 | A16057A | Neut |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Ultra-LEAF™ Purified anti-human IL-22
Recombinant human IL-22 (Black circles, Cat. No. 571306) sti...