- Clone
- BVD8-3G11 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Interleukin-3, Burst promoting activity, Eosinophil colony stimulating factor (Eo-CSF), Hematopoietic cell growth factor (HCGF), Mast (MGF/MCGF), Multi-colony stimulating (Multi-CSF), P cell stimulating activity (PCSA), Thy1 inducing factor
- Isotype
- Rat IgG1, κ
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Ultra-LEAF™ Purified anti-human IL-3 antibody inhibits the proliferation of TF-1 erythroleukemic cells induced by Recombinant Human IL-3 (carrier-free) (7 ng/ml) in a dose dependent manner, with an ED50 = 0.02 – 0.1 µg/ml.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 500506 | 500 µg | $325.00 | |||
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IL-3 is a highly species-specific pleiotropic factor produced primarily by activated T cells though also by mast cells, keratinocytes, and astrocytes, which stimulates colony formation of megakaryocytes, neutrophils, and macrophages from bone marrow cultures. The BVD8-3G11 antibody can neutralize the bioactivity of natural or recombinant IL-3.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Yeast-expressed, recombinant human IL-3
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
Block - Quality tested
IP, WB - Reported in the literature, not verified in house - Recommended Usage
-
Ultra-LEAF™ Purified anti-human IL-3 antibody inhibits the proliferation of TF-1 erythroleukemic cells induced by Recombinant Human IL-3 (carrier-free) (7 ng/ml) in a dose dependent manner, with an ED50 = 0.02 – 0.1 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
ELISA1-4 or ELISPOT Capture: The purified BVD8-3G11 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated BVD3-1F9 antibody (Cat. No. 500604) as the detecting antibody.
Additional reported applications include: Western blotting4 and immunoprecipitation4. -
Application References
(PubMed link indicates BioLegend citation) -
- Abrams JS, et al. 1992. Immunol. Rev. 127:5. (ELISA)
- Abrams JS et al. 1994. Eosnophil-active cytokines in human disease: development and use of monoclonal antibodies to il-3, il-5, and GM-CSF. In Eosinophils in Allergy and Inflammation. Gleich GJ and Kay AB, eds. Marcel Dekker, Inc. New York, NY. p. 133. (ELISA)
- Abrams J. 1995. Curr. Prot. Immunol. 6.20. (ELISA)
- Kaushansky K. 1992. J. Clin. Invest. 90:1879. (ELISA, IP, WB)
- Mahanty S, et al. 1992. J. Immunol. 148:3567.
- RRID
-
AB_2616770 (BioLegend Cat. No. 500506)
Antigen Details
- Structure
- Cytokine; 14-30 kD (Mammalian)
- Bioactivity
- Proliferation/differentiation of almost all types of hematopoietic progenitors into granulocytes, macrophages, erythroid cells, megakaryocytes, mast cell colonies; induces expression of 20-α-steroid dehydrogenase, histidine, ornithine decarboxylase.
- Cell Sources
- Activated T cells, mast cells, eosinophils, keratinocytes, NK cells, endothelial cells.
- Cell Targets
- Erythroid cells, megakaryocytes, neutrophils, eosinophils, basophils, mast cells, monocytic lineages.
- Receptors
- IL-3R, α subunit (CD123), common β subunit (CDw131).
- Biology Area
- Cell Biology, Stem Cells
- Molecular Family
- Growth Factors, Cytokines/Chemokines
- Antigen References
-
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Frendl G. 1992. Int. J. Immunopharmacol. 14:421.
3. Ihle J. 1992. Chem. Immunology 51:65. - Regulation
- Upregulated by T cell stimulation; downregulated by glucocorticoids.
- Gene ID
- 3562 View all products for this Gene ID
- UniProt
- View information about IL-3 on UniProt.org
Other Formats
View All IL-3 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human IL-3 | BVD8-3G11 | ELISA Capture,IP,WB |
| Ultra-LEAF™ Purified anti-human IL-3 | BVD8-3G11 | Block,IP,WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human IL-3
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Ultra-LEAF™ Purified anti-human IL-3
Ultra-LEAF™ Purified anti-human IL-3 antibody inhibits...