- Clone
- QA20A30 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- B cell growth factor 1 (BCGF-1), B-cell stimulatory factor 1 (BSF-1), interleukin-4, lymphocyte stimulatory factor 1
- Isotype
- Human IgG1, κ
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Recombinant human IL-4 (Cat. No. 766206) (black circles) induces the proliferation of TF-1 cells. Ultra-LEAF™ purified anti-human IL-4 recombinant antibody (clone QA20A30) (purple squares) inhibits the effect of recombinant human IL-4 in TF-1 cells in a dose-dependent manner whereas the Ultra-LEAF™ purified human IgG1 isotype ctrl recombinant antibody (Cat. No. 403502) (gray triangles) does not have the effect. ND50 range: 0.02 - 0.1 µg/mL.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 945303 | 100 µg | $358.00 | |||
| 945304 | 1 mg | $1205.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
IL-4 is the primary cytokine implicated in the development of Th2-mediated responses, which is associated with allergy and asthma. IL-4 binds to two receptor complexes, the Type I receptor comprises IL-4Rα and the common gamma-chain (γc), which is also shared by the cytokines IL-2, -7, -9, -15 and -21 and is present in hematopoietic cells. IL-4 can use the type II complex, comprising IL-4Rα and IL-13Rα1, which is present in non-hematopoietic cells. This second receptor complex is a functional receptor for IL-13, which shares approximately 25% homology with IL-4. The type I receptor complex can be formed only by IL-4 and is active in Th2 development. In contrast, the type II receptor complex formed by either IL-4 or IL-13 is more active during airway hypersensitivity and mucus secretion and is not found in T cells. IL-4, through its activation of STAT6, upregulates GATA3 expression and also suppresses TH1 and TH17 cell responses, partly through the upregulation of growth factor independent 1(GFI1), a transcriptional repressor of IFN-γ and IL-17 production. IL-4 induces macrophage activation and TSLP production. IL-4 recruits and activates IgE-producing B cells (IgE class switching) and enhances IgE-mediated responses by up-regulating IgE receptors on B lymphocytes, mast cells, and basophils. In addition, IL-4 also induces VCAM-1 on vascular endothelium and thus directs the migration of T lymphocytes, monocytes, basophils, and eosinophils to the inflammation site.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Recombinant
- Host Species
- Human
- Immunogen
- Recombinant human IL-4
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
Neut - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by the inhibition of proliferation induced by recombinant human IL-4 (Cat. No. 766206) on TF-1 cells. ND50 range: 0.02 - 0.1 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2892511 (BioLegend Cat. No. 945303)
AB_2892511 (BioLegend Cat. No. 945304)
Antigen Details
- Structure
- Heterodimer
- Distribution
-
IL-4 is produced by Th2 cells, naive CD4+ T cells, NKT cells, and basophils.
- Function
- Major role in differentiation of TH2 cells and induction of Th2 associated cytokines, suppresses TH1 and TH17 cell responses, induces macrophage activation and TSLP production, enhances IgE-mediated in B lymphocytes, mast cells, and basophils.
- Interaction
- T cells, B cells, macrophages, epithelial cells, smooth muscle cells, bronchial fibroblasts
- Ligand/Receptor
- IL-4 signals through Type I (IL-4Rα, γc) and Type II receptors (IL-4Rα, IL-13Rα1) complexes.
- Antigen References
-
- Swain SL, et al. 1990. J. Immunol. 145:3796-806.
- Hsieh CS, et al. 1992. Proc. Natl. Acad. Sci. USA 89:6065-9.
- Allison-Lynn A, et al. 2006. J. Immunol. 176:7456-61.
- Kato A, et al. 2007. J. Immunol. 179:1080-7.
- LaPorte SL, et al. 2008. Cell 132:259-72.
- Martinez FO, et al. 2009. Annu. Rev. Immunol. 27:451-83.
- Luzina IG, et al. 2012. J. Leuko. Biol. 92:753-64.
- Gene ID
- 3565 View all products for this Gene ID
- UniProt
- View information about IL-4 on UniProt.org
Other Formats
View All IL-4 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Ultra-LEAF™ Purified anti-human IL-4 Recombinant Antibody | QA20A30 | Neut |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Ultra-LEAF™ Purified anti-human IL-4 Recombinant Antibody
Recombinant human IL-4 (Cat. No. 766206) (black circles) ind...