- Clone
- MH9A3 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Interleukin-9, P40, Mast cell growth factor (MCGF), Megakaryoblast growth factor, T cell growth factor-3 (TCGF-3)
- Isotype
- Mouse IgG1, κ
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 507705 | 100 µg | $270.00 | |||
| 507706 | 1 mg | $743.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
IL-9 is a potent, T cell-derived, T cell growth factor which can also enhance mast cell activity and IL-3- or IL-4- dependent proliferation of bone marrow-derived mast cells. IL-9 synergizes with erythropoietin to promote erythroid colony formation. IL-9 has also been reported to protect human T cells from apoptosis induced by IL-2 withdrawal. IL-9 is upregulated in human eosinophils by TNF-α and IL1-β. IL-9 has been reported to downregulate the oxidative burst in activated human alveolar macrophages and induce TGF-β production. The MH9A3 antibody reacts with human IL-9. The MH9A3 antibody can inhibit IL-9 bioactivity in vitro.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Baculovirus-expressed, recombinant human IL-9
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
ELISA - Quality tested
Neut - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.5 - 2.0 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of IL-9 recombinant protein ranging from 1000 to 8 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
ELISA Detection4: The biotinylated MH9A3 antibody is useful as a detection antibody for a human IL-9 sandwich ELISA assay, when used in conjunction with Purified MH9A4 antibody (Cat. No. 507602) as the capture antibody.
Neutralization2,3: The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for neutralization of human IL-9 bioactivity (Cat. Nos. 507705 & 507706). -
Application References
(PubMed link indicates BioLegend citation) -
- Jenmalm M, et al. 2001. Clin. Exptl. Aller. 31:1528.
- Personal comunication with developer.
- Gounni AS, et. al, 2004. J. Immunol. 173:2771.
- Faulkner H, et al. 2002. J. Infec. Diseas. 185:665.
- RRID
-
AB_2890853 (BioLegend Cat. No. 507705)
AB_2890853 (BioLegend Cat. No. 507706)
Antigen Details
- Structure
- Cytokine; 32-39 kD (Mammalian)
- Bioactivity
- Potentiates production of IgG, IgM, and IgE by IL-4-induced B lymphocytes; regulates granzyme family proteases; enhances proliferation of bone marrow mast cells with IL-3; production of IL-6 by mast cells
- Cell Sources
- IL-2 activated TH2 lymphocytes, Hodgkin's lymphoma
- Cell Targets
- T lymphocytes, erythroid precursors
- Receptors
- IL-9R
- Biology Area
- Immunology
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Quesniaux V. 1992. Research Immunology 143:385.
3. Renauld J, et al. 1993. Adv. Immunol. 54:79.
4. Yang Y. 1992. Leuk. Lymphoma 8:441. - Regulation
- Upregulated by IL-1 after TCR activation, TNF-α; downregulated by antibodies to IL-2
- Gene ID
- 3578 View all products for this Gene ID
- UniProt
- View information about IL-9 on UniProt.org
Other Formats
View All IL-9 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Ultra-LEAF™ Purified anti-human IL-9 | MH9A3 | ELISA,Neut |
| Brilliant Violet 421™ anti-human IL-9 | MH9A3 | ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Ultra-LEAF™ Purified anti-human IL-9
-
Brilliant Violet 421™ anti-human IL-9
Th2-polarized human lymphocytes were stimulated with Cell Ac...