- Clone
- A16067H (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Macrophage Colony-Stimulating Factor 1, MCSF, CSF-1, Macrophage Colony Stimulating Factor 1, Colony Stimulating Factor 1 (Macrophage)
- Isotype
- Mouse IgG2b, κ
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Recombinant human M-CSF induces the proliferation of mouse myelogenous leukemia lymphoblast M-NFS-60 cells in a dose dependent manner (black triangles). Anti-human M-CSF monoclonal antibody (clone A15139F, purple circles) neutralizes the proliferation of M-NFS-60 cells induced by recombinant human M-CSF (10 ng/ml). The ND50 = 0.01 – 0.05 µg/ml.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 699203 | 100 µg | $270.00 | |||
| 699204 | 1 mg | $715.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
M-CSF was first characterized as a glycoprotein that induces monocyte and macrophage colony formation from precursors in murine bone marrow cultures. M-CSF is constitutively present at biologically active concentrations in human serum. It binds CD14+ monocytes and promotes the survival/proliferation of human peripheral blood monocytes. In addition, M-CSF enhances inducible monocyte functions including phagocytic activity, microbial killing, cytotoxicity for tumor cells as well as synthesis of inflammatory cytokines such as IL-1, TNFα, and INFγ in monocytes. M-CSF induces RANKL production in mature human osteoclasts; consequently, M-CSF is a potent stimulator of mature osteoclast resorbing activity. Also, M-CSF induces VEGF in human monocytes in human tumors; high levels of M-CSF, mononuclear phagocytes, and VEGF are associated with poor prognosis in patients with cancer. High levels of M-CSF have been associated with different pathologies such as pulmonary fibrosis and atherosclerosis. M-CSF binds to its receptor M-CSFR, and this receptor is shared by a second ligand, IL-34. Human M-CSF and IL-34 exhibit cross-species specificity, both bind to human and mouse M-CSF receptors.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human M-CSF
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
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Neut - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by neutralizing activity. The ND50 is 0.01 - 0.05 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
-
- RRID
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AB_2715880 (BioLegend Cat. No. 699203)
AB_2715880 (BioLegend Cat. No. 699204)
Antigen Details
- Structure
- Disulfide-linked glycosylated homodimer
- Distribution
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M-CSF is released by fibroblasts, breast cancer cell lines, alveolar macrophages, stromal bone marrow cells, endothelial cells, and mesenchimal cells.
- Function
- Key regulator of the survival, proliferation, and differentiation of mononuclear phagocytes and plays a central role in the regulation of osteoclastogenesis. Regulates the development of Paneth cells, Langerhans cells, lamina propria dendritic cells, and microglia.
- Interaction
- Monocytes, macrophages, mononuclear phagocyte precursors, microglia, proliferating smooth muscle cells, umbilical vein endothelial cells, and breast cancer cell lines.
- Ligand/Receptor
- M-CSFR or CSF1R (CD115)
- Biology Area
- Cell Biology, Cell Proliferation and Viability, Immunology
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Antigen References
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1. Kawasaki ES, et al. 1985. Science 230:291.
2. Wei S, et al. 2010. J. Leuko. Biol. 88:495.
3. Hodge JM, et al. 2011. PloS One 6:e21462.
4. Morandi A, et al. 2011. PloS One 6:e27450.
5. Erblich B, et al. 2011. PloS One 6:e26317.
6. MacDonald KP, et al. 2010. Blood 116:3955. - Gene ID
- 1435 View all products for this Gene ID
- UniProt
- View information about M-CSF on UniProt.org
Other Formats
View All M-CSF Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Ultra-LEAF™ Purified anti-human M-CSF | A16067H | Neut |
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Ultra-LEAF™ Purified anti-human M-CSF
Recombinant human M-CSF induces the proliferation of mouse m...