- Clone
- QA18A10 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- TGFbeta,TGFB, TGF-Beta-1, TGF-Beta1
- Isotype
- Mouse IgG1, κ
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Recombinant human TGF-β1 (Cat. No. 781802) (black circles) inhibits the proliferation of HT-2 cells induced by recombinant mouse IL-4 at 5 ng/mL (Cat. No. 574302). Ultra-LEAF™ Purified anti-human/mouse TGF-β1 (clone QA18A10) (purple circles) inhibits the effect of recombinant human TGF-β1 (at 0.4 ng/mL) in mouse HT-2 cells in a dose-dependent manner; whereas the mouse IgG1, κ isotype control (grey triangles, clone MOPC-21, Cat. No. 400165) does not have effect. The ND50 = 0.05 – 0.25 µg/mL
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 947303 | 100 µg | $358.00 | |||
| 947304 | 1 mg | $1205.00 | |||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
TGF-β1 is synthesized in cells as a 390-amino acid. Furin cleaves the protein at residue 278, yielding an N-terminal cleavage product which corresponds to the latency-associated peptide (LAP), and the 25-kD C-terminal portion of the precursor constitutes the mature TGF-β1. TGF-β1 activators can release TGF-β1 from LAP. These activators include proteases that degrade LAP, thrombospondin-1, reactive oxygen species, and integrins avb6 and avb8. Mouse TGF-β1 converts naïve T cells into regulatory T (Treg) cells that prevent autoimmunity. Although human TGF-β1 is widely used for inducing FOXP3+ in vitro, it might not be an essential factor for human Treg differentiation. Th17 murine can be induced from naïve CD4+ T cells by the combination of TGF-β1 and IL-6 or IL-21. Nevertheless, the regulation of human Th17 differentiation is distinct. TGF-β1 seems to have dual effects on human Th17 differentiation in a dose-dependent manner. While TGF-β1 is required for the expression of RORγt, in human naive CD4+ T cells from cord blood, TGF-β1 can inhibit the function of RORγt at high doses. By using serum-free medium, it has been clarified that the optimum conditions for human Th17 differentiation are TGF-β1, IL-1β, and IL-2 in combination with IL-6, IL-21 or IL-23.
Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Immunogen
- Human TGF-β1
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
Neut - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by neutralizing the inhibition of HT-2 cell proliferation induced by recombinant human TGF-β1 (0.4 ng/mL, Cat. No. 781802). ND50 range: 0.05 – 0.25 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2910510 (BioLegend Cat. No. 947303)
AB_2910510 (BioLegend Cat. No. 947304)
Antigen Details
- Structure
- Homodimer
- Distribution
-
TGF-β1 is secreted by numerous cells
- Function
- Regulation of cell growth and survival, cell and tissue differentiation, development, inflammation, immunity, hematopoiesis, and tissue remodeling and repair. Essential for wound healing, stimulates matrix molecule deposition and angiogenesis, and is an essential mediator of the pathologic scarring in fibrotic disorders
- Interaction
- Embryonic stem cells, endothelial cells, fibroblasts, mesenchymal cells, mesenchymal stem cells, neural stem cells, osteoclasts, Th17, Tregs
- Ligand/Receptor
- TGF-β1 binds to type II and type I serine/threonine kinase receptors, which initiate intracellular signals through activation of Smad proteins.
- Cell Type
- Tregs
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Antigen References
-
- Sun X, et al. 2013. J Virol. 87:10126-38.
- Benlhabib H, et al. 2015. J Biol Chem. 290:22409-22.
- Lin C, et al. 2016. J Exp Med. 213:251-71.
- Chen P, et al. 2016. Sci Rep. 6:33407.
- de la Mare J, et al. 2017. BMC Cancer. 17:202.
- Du C, et al. 2017. Int J Biochem Cell Biol. 90:17-28.
- Nüchel J, et al. 2018. Autophagy. 14:465-486.
- Gene ID
- 7040 View all products for this Gene ID
- UniProt
- View information about TGF-beta1 on UniProt.org
Other Formats
View All TGF-β1 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Ultra-LEAF™ Purified anti-human/mouse TGF-β1 Recombinant Antibody | QA18A10 | Neut |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Ultra-LEAF™ Purified anti-human/mouse TGF-β1 Recombinant Antibody
Recombinant human TGF-β1 (Cat. No. 781802) (black circles) i...